Molecular detection of mutations involved in Helicobacter pylori antibiotic resistance in Algeria

The Journal of Antimicrobial Chemotherapy
Meryem BachirKara Turki Douidi

Abstract

In Algeria, there are limited data regarding the pattern of Helicobacter pylori primary antibiotic resistance. The aim of this study was to evaluate the primary resistance of H. pylori to clarithromycin, ciprofloxacin, tetracycline and rifampicin and to determine the molecular mechanisms involved in the resistance. Two hundred and seventy Algerian adults who had never received H. pylori treatment were enrolled in this study. Human biopsies were obtained for culture and antimicrobial susceptibility testing was performed by Etest for clarithromycin, ciprofloxacin, tetracycline and rifampicin. Real-time fluorescence resonance energy transfer (FRET)-PCR was also performed in all cases to assess primary clarithromycin resistance and point mutations involved, real-time PCR was used to detect mutations involved in tetracycline primary resistance and sequencing of the QRDR of gyrA was performed to detect mutations involved in quinolone resistance. No resistance to rifampicin was detected. Resistance to clarithromycin and ciprofloxacin was found in 29.7% and 17.9%, respectively. Results of real-time FRET-PCR showed that A2143G was the most frequent point mutation, A2142C was not found and 42 patients (15.5%) were infected by both resist...Continue Reading

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Citations

Sep 6, 2019·Helicobacter·Anthony O'ConnorColm O'Morain
Sep 6, 2019·Helicobacter·Athanasios MakristathisEmilie Bessède
Oct 18, 2020·Helicobacter·Dirk HofreuterErik-Oliver Glocker
Jun 19, 2019·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·B BluemelE-O Glocker
Feb 16, 2021·Archives of Medical Research·Patricio González-HormazábalArnoldo Riquelme
Mar 2, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Chuanfeng ZhangLinfu Zhou
Jun 15, 2021·Arab Journal of Gastroenterology : the Official Publication of the Pan-Arab Association of Gastroenterology·Yonglin LiXiuling Wu

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