Molecular detection of Mycobacterium ulcerans in the environment and its relationship with Buruli ulcer occurrence in Zio and Yoto districts of maritime region in Togo

PLoS Neglected Tropical Diseases
Issaka MamanGisela Bretzel

Abstract

Buruli Ulcer (BU) is a neglected tropical skin infection caused by Mycobacterium ulcerans. Residence near aquatic areas has been identified as an important source of transmission of M. ulcerans with increased risk of contracting Buruli ulcer. However, the reservoir and the mode of transmission are not yet well known. The aim of this study was to identify the presence of M. ulcerans in the environment and its relationship with Buruli ulcer occurrence in Zio and Yoto districts of the maritime region in south Togo. A total of 219 environmental samples including soil (n = 119), water (n = 65), biofilms/plants (n = 29) and animals' feces (n = 6) were collected in 17 villages of Zio and Yoto districts of the maritime region in Togo. DNA of M. ulcerans including IS2404 and IS2606 insertions sequences and mycolactone ketoreductase-B gene (KR-B) was detected using real time PCR amplification (qPCR) technique. In parallel, clinical samples of patients were tested to establish a comparison of the genetic profile of M. ulcerans between the two types of samples. A calibration curve was generated for IS2404 from a synthetic gene of M. ulcerans Transposase pMUM001, the plasmid of virulence. In the absence of inhibition of the qPCR, 6/219 (2.7...Continue Reading

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Citations

Jul 25, 2019·The American Journal of Tropical Medicine and Hygiene·Nassim HammoudiAmar Bouam
Nov 22, 2018·Current Tropical Medicine Reports·Rie R YotsuKingsley Asiedu
Jul 24, 2020·Microbial Pathogenesis·N HammoudiM Drancourt
Aug 27, 2021·PLoS Neglected Tropical Diseases·Anthony J MuletaChris Greening

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Datasets Mentioned

BETA
IS2404

Methods Mentioned

BETA
PCR

Software Mentioned

SPSS Statistical Package for Social Science
SPSS

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