Molecular detection of rabies encephalitis and correlation with cytokine expression

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Gerard J NuovoYilan Zhang

Abstract

The purposes of this study were to elucidate the role of cytokine upregulation in the pathogenesis of rabies encephalitis and to compare the detection of Negri bodies with that of rabies protein by immunohistochemistry and rabies RNA by reverse transcriptase (RT) in situ PCR for its diagnosis. Negri bodies were evident in 4/7 of the documented rabies cases; viral protein and viral RNA were detected in each case. The average number of rabies-infected cells, determined by counting 150 neurons in serial sections in areas where viral protein was evident, with the three different detection methods was: Negri bodies (<1/150), immunohistochemistry (4/150), and RT in situ PCR (49/150). No rabies protein or RNA was detected in four control brain tissues that were read with the rabies cases in a blinded fashion. The ratio of cells expressing tumor necrosis factor alpha (TNFalpha) or inducible nitric oxide synthetase (iNOS) to 1 SSI-1/SOCS-1 (suppressors of cytokine signaling) expression, which is a novel class of negative feedback regulators of cytokine receptor signaling, was markedly increased only in the areas where many viral infected cells were present. Colabeling experiments showed that most of the cells expressing iNOS or TNFalpha...Continue Reading

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Citations

Apr 7, 2010·Vaccine·Nicholas JohnsonAnthony R Fooks
Mar 8, 2008·Annals of Diagnostic Pathology·Fernando GabilondoHugo Domínguez-Malagón
Sep 24, 2015·Virusdisease·S MehtaA Chowdhary
Feb 15, 2015·Current Opinion in Virology·Phillip A Swanson, Dorian B McGavern
Jul 1, 2008·Advances in Virus Research·Susan A Nadin-Davis, Christine Fehlner-Gardiner
Jun 1, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Daniele M BassuinoDavid Driemeier
Nov 24, 2006·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Fabiane Carvalho de MacedoGerard J Nuovo
Jan 30, 2007·Veterinary Research Communications·P Ezhil PraveenaB Murali Manohar
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Timothy W PharesD Craig Hooper
Dec 20, 2018·Neurology. Clinical Practice·Jonathan H SmithMauricio F Villamar
Jan 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Manoj ThapaDaniel J J Carr
Dec 20, 2018·Neurology. Clinical Practice·Alan C Jackson, Marc R Del Bigio
Jul 13, 2006·Archives of Pathology & Laboratory Medicine·Gerard Nuovo

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