PMID: 11368106May 23, 2001Paper

Molecular detection of respiratory syncytial virus in postmortem lung tissue samples from Mexican children deceased with pneumonia

The Pediatric Infectious Disease Journal
M E Bustamante-CalvilloO Muñoz-Hernández

Abstract

Respiratory syncytial virus (RSV) is the major viral cause of severe respiratory infections in children younger than 2 years of age. Nevertheless there are not enough epidemiologic data about the role of RSV as a cause of infantile mortality from pneumonia, mainly in young children from developing countries Aim. To determine the frequency of RSV infection in lung tissue samples from Mexican children deceased with pneumonia, by reverse transcription (RT) and PCR. Postmortem lung tissue samples from 98 children younger than 2 years of age who died of pneumonia during the period of 1989 to 1997 were studied. Paraffin was removed with xylene from 10-microm lung sections, the total RNA was extracted and complementary DNA was obtained by RT reaction. A nested PCR with the use of oligonucleotides specific for the F glycoprotein gene was developed. Samples negatives for RSV were tested for the absence of polymerase inhibitors and for complementary DNA integrity. Twenty-nine of the 98 (30%) children deceased with pneumonia were positive for RSV by RT-PCR; 8 were detected from 13 (62%) children with histopathologic diagnosis of viral pneumonia and 21 from 85 (25%) children with histopathologic diagnosis of bacterial pneumonia (P = 0.018)...Continue Reading

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Citations

Nov 30, 2002·Paediatric Respiratory Reviews·Gilberto Bueno FischerAlejandro J Colom
Aug 2, 2002·The Pediatric Infectious Disease Journal·P A Piedra
Feb 12, 2004·The Pediatric Infectious Disease Journal·Daniel E NoyolaJ Raúl Ochoa-Zavala
Oct 20, 2009·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Daniel LevinJohn F Modlin
Jan 19, 2012·BMC Infectious Diseases·Jamal A AhmedRachel B Eidex
Jun 12, 2008·Bulletin of the World Health Organization·Igor RudanHarry Campbell
Dec 22, 2010·Paediatric Respiratory Reviews·Varinder Singh, Satinder Aneja
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Feb 21, 2018·Pediatric Pulmonology·Mauricio T Caballero, Fernando P Polack
Mar 26, 2005·Canadian Journal of Physiology and Pharmacology·Gordon BolgerMichel Liuzzi
May 25, 2012·Primary Care Respiratory Journal : Journal of the General Practice Airways Group·Marianne Stubbe ØstergaardRune Aabenhus
May 29, 2019·Pediatrics International : Official Journal of the Japan Pediatric Society·Tomoya IkedaTakaki Ishikawa

Related Concepts

Autopsy
Prevalence Studies
Lung
Respiratory syncytial virus
Retrospective Studies
RNA, Viral
Nested Polymerase Chain Reaction
Respiratory Syncytial Virus Infections
Pneumonia, Bacterial

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