Molecular detection of Rickettsia felis in common fleas in Greece and comparative evaluation of genotypic methods.

Journal of Microbiological Methods
Georgios DougasJoseph Papaparaskevas

Abstract

Rickettsia felis is the causative agent of flea-borne spotted fever (FBSF), an emerging zoonosis. Although there is evidence of FBSF in Greece, fleas, the classic vectors of R. felis, have not been adequately studied. Thus, the aim of this study was to detect and characterize bacteria of genus Rickettsia and especially R. felis from common fleas parasitizing domestic cats and dogs in Greece and evaluate the efficiency of established molecular techniques. DNA of flea-pools (samples) by animal-host was investigated by quantitative real-time PCRs (qPCR), and 16S metagenomics (16S). Determination of Rickettsia spp., Rickettsia felis-like organisms (RFLOs), and R. felis was based on a combination of qPCRs targeting gltA and ompB genes, 16S automated metagenomics and manual comparison of 16S sequences for >99% similarity with the publicly available 16S R. felis GenBank sequences using the Basic Local Alignment Search Tool (BLAST>99). Information for the animal-hosts was available and statistically analyzed. Among 100 flea-pools, R. felis was detected in 14 samples with a combination of six, five and three assays in 10, two and two samples, respectively. The sensitivity of the assays for Rickettsia genus (16S, and genus specific qPCRs...Continue Reading

References

May 12, 2009·Parasites & Vectors·Gioia CapelliDomenico Otranto
Jul 1, 2010·Emerging Infectious Diseases·Cristina SocolovschiDidier Raoult
Oct 15, 2011·Vector Borne and Zoonotic Diseases·Cornelia SilaghiSteffen Rehbein
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Oct 29, 2017·Acta Tropica·Dimosthenis ChochlakisAnna Psaroulaki
Apr 13, 2019·Parasites & Vectors·Lavel Chinyama MoongaJunya Yamagishi

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