Molecular Detection of Theileria spp. in Livestock on Five Caribbean Islands

BioMed Research International
Jilei ZhangChengming Wang

Abstract

Theileria spp. are tick-transmitted, intracellular apicomplexan protozoan parasites infecting a wide range of animals. As there is very limited information on the prevalence of Theileria spp. in the Caribbean we used the recently described genus-specific pan-Theileria FRET-qPCR to identify infected animals in the region and a standard 18S rRNA gene PCR and sequencing to determine the species involved. We found Theileria spp. in 9% of the convenience samples of animals (n = 752) studied from five Caribbean islands. Donkeys (20.0%: 5/25) were most commonly infected, followed by sheep (17.4%, 25/144), cattle (6.8%; 22/325), goats (5.0%; 12/238), and horses (5.0%; 1/20). Six species of Theileria were identified: T. equi (donkeys, cattle, goats, and sheep), Theileria sp. OT3 (sheep and goats), Theileria sp. NG-2013a (cattle), Theileria sp. YW-2014 (donkeys), Theileria sp. B15a (goats), and Babesia vulpes or a closely related organism (sheep and goats). Only T. equi has been previously reported in the Caribbean. Our findings expand the known host ranges of Theileria spp. and the known distribution of the organisms around the world.

References

Dec 1, 1985·Veterinary Parasitology·G I Garris, K Scotland
Mar 1, 1995·Veterinary Parasitology·E Camus, N Barre
May 15, 1999·Journal of Clinical Microbiology·J M GubbelsF Jongejan
Jun 5, 2003·Veterinary Parasitology·Joanne RampersadDavid Ammons
Nov 23, 2006·Veterinary Parasitology·Zinora AsgaraliErnest Caesar
Apr 5, 2008·Innate Immunity·Jabbar S AhmedUlrike Seitzer
Nov 6, 2010·Veterinary Parasitology·Karla C GeorgesMichael J Yabsley
Dec 17, 2010·The Journal of Parasitology·Patrick KellyJohn Dame
May 22, 2013·Parasitology Research·Mehmet Fatih AydinNazir Dumanli
Sep 17, 2013·Journal of Veterinary Internal Medicine·L N WiseD P Knowles
Jan 7, 2014·Parasitology International·Zhancheng TianJin Luo
Mar 26, 2014·Parasites & Vectors·Mathew AdamuPaul T Matjila
May 3, 2014·Parasitology International·Saw BawmKen Katakura
Aug 8, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Thillaiampalam SivakumarNaoaki Yokoyama
Aug 15, 2014·Acta Parasitologica·Lawrence P BelotindosClaro N Mingala
Jan 8, 2015·Annual Review of Entomology·Glen A Scoles, Massaro W Ueti
Feb 17, 2015·Journal of the South African Veterinary Association·Chantal T RosaEran Dvir
Apr 2, 2015·International Journal for Parasitology. Parasites and Wildlife·Ben J MansAbdalla A Latif
Apr 19, 2015·Parasites & Vectors·Gad BanethLeonhard Schnittger

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Citations

Dec 15, 2017·Frontiers in Cellular and Infection Microbiology·Mathilde GondardSara Moutailler
Aug 15, 2018·The Journal of Parasitology·Zheng-Qing YuGuang-Hui Zhao
Aug 1, 2020·Pathogens·Dasiel Obregón AlvarezAlejandro Cabezas-Cruz
Jul 24, 2018·Parasitology Research·Adrian Alberto Díaz-SánchezBelkis Corona-González
Aug 11, 2019·Scientific Reports·Kifaya AzmiZiad Abdeen
Dec 4, 2020·Animals : an Open Access Journal From MDPI·Snorre Stuen

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Datasets Mentioned

BETA
KF773740

Methods Mentioned

BETA
PCR
PCRs

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