Molecular detection of two major gastrointestinal parasite genera in cattle using a novel droplet digital PCR approach

Parasitology Research
Paulius BaltrušisJohan Höglund

Abstract

Cooperia sp. and Ostertagia sp. are two cosmopolitan parasitic nematodes often found in mixed gastrointestinal infections in cattle across temperate regions. In light of the recent increase in the emergence of anthelmintic resistance in these and other nematodes derived from cattle around the globe, and their negative impact on animal health and productivity, novel molecular assays need to be put forth in order to facilitate the monitoring of parasite burden in infected herds, using pasture and/or fecal samples. Here, we describe a novel droplet digital PCR platform-based concept for precise identification and quantification of the two most abundant and important parasite genera in grazing western European cattle. By exploiting a single nucleotide difference in the two parasites' ITS2 sequence regions, we have developed two specific hydrolysis probes labeled with FAM™ or HEX™ fluorophores, which can not only distinguish between the DNA sequences of the two, but also quantify them in mixed DNA samples. A third, newly developed universal probe was also tested along the genus-specific probes to provide a robust and accurate reference. It was evident that the universal probe displayed congruent results to those obtained by the genu...Continue Reading

References

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May 15, 2018·International Journal for Parasitology. Drugs and Drug Resistance·Safaa T ElmahalawyJohan Höglund
Sep 29, 2018·International Journal for Parasitology. Drugs and Drug Resistance·Paulius BaltrušisJohan Höglund

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Citations

Oct 1, 2020·The Journal of Parasitology·Zhichao YuXianghong Ju
Apr 3, 2021·International Journal for Parasitology. Drugs and Drug Resistance·Paulius BaltrušisJohan Höglund
Jun 7, 2021·Animal : an International Journal of Animal Bioscience·Niclas HögbergJohan Höglund

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Methods Mentioned

BETA
PCR

Software Mentioned

CodonCode Aligner
ggplot2
R
QuantaSoft

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