Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis.

Applied and Environmental Microbiology
Gerard A CangelosiJohn S Meschke

Abstract

Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.

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Citations

May 6, 2011·Journal of Clinical Microbiology·Peter E VandeventerAngelika Niemz
Apr 3, 2013·Journal of Microbiological Methods·Volker Gürtler, Danilla Grando
Sep 21, 2013·Journal of Microbiological Methods·Volker Gürtler, Danilla Grando
May 13, 2014·The Journal of Molecular Diagnostics : JMD·Pablo González-AndradeStijn Deborggraeve
Jul 25, 2013·Mucosal Immunology·J A Platts-MillsE R Houpt
Aug 20, 2017·Applied and Environmental Microbiology·Cecilia M ChiriacCristian Coman
Mar 12, 2017·Applied and Environmental Microbiology·Kris M WeigelGerard A Cangelosi
Jul 20, 2014·Applied and Environmental Microbiology·Gerard A Cangelosi, John S Meschke
Mar 8, 2019·Microbiology·Shravanthi S Kumar, Asit Ranjan Ghosh
Sep 22, 2019·Scientific Reports·Andreia Patrícia MagalhãesNuno Cerca
Nov 18, 2018·Folia Microbiologica·Lisa-Maria MauerhoferSimon K-M R Rittmann
Nov 17, 2016·Frontiers in Microbiology·Francis HassardShelagh K Malham
Nov 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Diana C VanegasEric S McLamore
Jul 28, 2021·Current Protocols·Kris M WeigelGerard A Cangelosi
Jul 17, 2012·Environmental Science & Technology·Ameet J PintoLutgarde Raskin

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