Molecular detection of virulence markers to identify diarrhoeagenic Escherichia coli isolated from Mula-Mutha river, Pune District, India.

Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
Sivanandan R NamachivayamTannaz Birdi

Abstract

In this study presence of virulence genes in multidrug resistant Escherichia coli isolated from Mula-Mutha river, Pune, India was undertaken. The objective was to understand whether the isolates were of diarrhoeagenic or of environmental origin. This was essential since the river flows through urban and rural parts of Pune and its water is used not only for industrial and agricultural purposes but also for domestic usage. One hundred and two multidrug E. coli isolates were selected from our previous study which detected genes coding for antibiotic resistance as well as identified integrons associated with multidrug resistance. Isolates were subjected to multiplex PCR to detect presence of virulence genes, set1A, set1B, sen astA, aggA, aafA, pet, stx1 and stx. Sequencing was performed to confirm the amplified PCR product. Seven of the 102 E. coli isolates showed gene set1A alone identifying them as Enteroaggregative E. coli. Thus, the findings revealed that majority of drug resistant E. coli were environmental in origin. The presence of antibiotic resistant genes, integrons in the environment as well as diarrhoeagenic E. coli isolates is a warning and calls for efficient public health measures to ensure that untreated sewage and...Continue Reading

References

Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Nov 9, 2000·The Journal of Infectious Diseases·J VilaJ P Nataro
May 22, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J A AdachiH L DuPont
Jun 9, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·George A Jacoby
Mar 16, 2006·Trends in Microbiology·Craig Baker-AustinJ V McArthur
Jun 7, 2008·Current Opinion in Biotechnology·Fernando BaqueroRafael Cantón
Sep 18, 2010·Diagnostic Microbiology and Infectious Disease·Priya RajendranGagandeep Kang
May 15, 2012·Lancet·Li LiuUNKNOWN Child Health Epidemiology Reference Group of WHO and UNICEF
Apr 4, 2013·Mobile Genetic Elements·Sara DominguesKaare M Nielsen
Apr 16, 2013·Lancet·Christa L Fischer WalkerRobert E Black
May 28, 2014·Nature Reviews. Microbiology·Dan I Andersson, Diarmaid Hughes
Mar 31, 2015·Nature Reviews. Microbiology·Thomas U BerendonkJosé Luis Martinez
Oct 30, 2016·The Lancet Infectious Diseases·Ammara Mushtaq
Dec 31, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anthony Ayodeji AdegokeThor Axel Stenström
Jun 14, 2018·International Journal of Environmental Research and Public Health·Rutuja DhawdeTannaz Birdi
Aug 22, 2018·Environmental Monitoring and Assessment·Rutuja DhawdeTannaz Birdi
Aug 24, 2018·Environmental Science and Pollution Research International·Thirumurthy VelpandianShehnaz P Batta
Dec 12, 2018·Journal of Environmental Management·Vijay Bahadur YadavSippy Kalra
Nov 16, 2019·Microbial Cell Factories·Leticia ArreguiNorma A Valdez-Cruz

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

MEGA6
ClustalW

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Environmental Research and Public Health
Rutuja DhawdeTannaz Birdi
Eastern Mediterranean Health Journal = La Revue De Santé De La Méditerranée Orientale = Al-Majallah Al-ṣiḥḥīyah Li-sharq Al-mutawassiṭ
B PourakbariS Mamishi
Scandinavian Journal of Infectious Diseases
Asem A ShehabiKamal G Hajjaj
Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Megha KaushikPooja Gulati
© 2021 Meta ULC. All rights reserved