Molecular determinants dictating cell surface expression of the human sodium-dependent vitamin C transporter-2 in human liver cells.

American Journal of Physiology. Gastrointestinal and Liver Physiology
Veedamali S SubramanianHamid M Said

Abstract

The human sodium-dependent vitamin C transporter-2 (hSVCT2) plays an important role in cellular accumulation of ascorbic acid in liver cells. However, little is known about the molecular determinants that direct hSVCT2 to the cell surface in hepatocytes. We addressed this issue using live cell imaging methods to resolve the distribution and trafficking of truncated or mutated hSVCT2 constructs in a cellular model of human hepatocytes, HepG2 cells. Whereas a full-length hSVCT2-yellow fluorescent protein (YFP) fusion protein was functionally expressed at the cell surface in HepG2 cells, serial truncation and mutation analysis demonstrated an essential role for both NH(2)- and COOH-terminal sequence(s) for cell surface expression and function. Video-rate confocal imaging showed evidence of dynamic hSVCT2-YFP containing intracellular trafficking vesicles, the motility of which was impaired following disruption of microtubules using nocodazole. However, in a HepG2 cell line stably expressing hSVCT2-YFP at the cell surface, plasma membrane levels of hSVCT2 were unaffected by inhibition of microtubule-associated motor proteins; rather, surface expression of hSVCT2-YFP was increased following treatment with myosin inhibitors. Together,...Continue Reading

References

Jun 5, 1999·The American Journal of Clinical Nutrition·A C Carr, B Frei
Jul 1, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J A DranoffM H Nathanson
Sep 2, 1999·Biochemical and Biophysical Research Communications·D P RajanP D Prasad
Jun 9, 2001·Molecular Membrane Biology·W J LiangS M Jarvis
Nov 28, 2002·The Journal of Biological Chemistry·Veedamali S SubramanianHamid M Said
Jul 8, 2003·Pflügers Archiv : European journal of physiology·Hitomi TakanagaMatthias A Hediger
Nov 26, 2003·The American Journal of Gastroenterology·Stephen A HarrisonSteven Schenker
Jul 5, 2005·Biochemical and Biophysical Research Communications·James C BoyerShiu-Ming Kuo
Sep 2, 2005·American Journal of Physiology. Cell Physiology·Tatemitsu RaiShinichi Uchida
Dec 24, 2005·The Journal of Biological Chemistry·Veedamali S SubramanianHamid M Said
Mar 28, 2006·The Journal of Nutritional Biochemistry·Vanessa M D'SouzaPeter W Swaan
Dec 22, 2006·American Journal of Physiology. Renal Physiology·Hong C LiManoocher Soleimani
Jun 2, 2007·Amino Acids·I SaviniM V Catani
Sep 22, 2007·The Journal of Nutrition·Yi Li, Herb E Schellhorn
May 3, 2008·The Journal of Physiology·Michael J Boulware, Jonathan S Marchant
Jul 16, 2008·Biochemical and Biophysical Research Communications·Veedamali S SubramanianHamid M Said
Oct 11, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jack C ReidlingHamid M Said
Feb 13, 2009·American Journal of Physiology. Cell Physiology·Veedamali S SubramanianHamid M Said
Apr 28, 2009·Journal of Physiology and Biochemistry·C I RivasJ C Vera
Jul 3, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Balasubramaniem AshokkumarHamid M Said

❮ Previous
Next ❯

Citations

May 5, 2012·PloS One·Alessandro PecciUNKNOWN Italian Registry for MYH9-releated diseases
Mar 20, 2013·Molecular Aspects of Medicine·Marc BürzleMatthias A Hediger
Dec 7, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Eduardo Jiménez-FernándezCarlos Infante
May 10, 2017·Frontiers in Oncology·Christina WohlrabGabi U Dachs

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.