Molecular Determinants of Thyroid Nodules with Indeterminate Cytology and RAS Mutations.

Thyroid : Official Journal of the American Thyroid Association
Juan C Hernandez-PreraChristine H Chung

Abstract

Background: RAS gene family mutations are the most prevalent in thyroid nodules with indeterminate cytology and are present in a wide spectrum of histological diagnoses. We evaluated differentially expressed genes and signaling pathways across the histological/clinical spectrum of RAS-mutant nodules to determine key molecular determinants associated with a high risk of malignancy. Methods: Sixty-one thyroid nodules with RAS mutations were identified. Based on the histological diagnosis and biological behavior, the nodules were grouped into five categories indicating their degree of malignancy: non-neoplastic appearance, benign neoplasm, indeterminate malignant potential, low-risk cancer, or high-risk cancer. Gene expression profiles of these nodules were determined using the NanoString PanCancer Pathways and IO 360 Panels, and Angiopoietin-2 level was determined by immunohistochemical staining. Results: The analysis of differentially expressed genes using the five categories as supervising parameters unearthed a significant correlation between the degree of malignancy and genes involved in cell cycle and apoptosis (BAX, CCNE2, CDKN2A, CDKN2B, CHEK1, E2F1, GSK3B, NFKB1, and PRKAR2A), PI3K pathway (CCNE2, CSF3, GSKB3, NFKB1, PPP2...Continue Reading

References

Sep 15, 1992·Cancer·K R Cho, B Vogelstein
Aug 18, 1994·Nature·P Rodriguez-VicianaJ Downward
Feb 25, 1993·The New England Journal of Medicine·E L Mazzaferri
Jan 16, 2002·American Journal of Clinical Pathology·Zubair W Baloch, Virginia A Livolsi
Aug 13, 2004·The Journal of Pathology·Catherine R Tait, Pamela F Jones
Sep 17, 2004·The American Journal of Surgical Pathology·Ricardo V LloydMarick E Lae
Jun 2, 2005·The Journal of Clinical Endocrinology and Metabolism·Guojun WuMingzhao Xing
Apr 12, 2007·The Journal of Clinical Endocrinology and Metabolism·Yangang WangMingzhao Xing
May 17, 2007·Chest·Joo Hun ParkSung Chul Hwang
Jun 24, 2008·Nature Reviews. Molecular Cell Biology·Antoine E Karnoub, Robert A Weinberg
Sep 12, 2008·The New England Journal of Medicine·Robert I Haddad, Dong M Shin
Feb 5, 2009·Thyroid : Official Journal of the American Thyroid Association·Michael RiveraRonald A Ghossein
Feb 21, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Iris HelfrichHellmut G Augustin
Jun 8, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Michael RiveraRonald A Ghossein
Jul 24, 2010·Nature Reviews. Cancer·Hanhua HuangRodney Lappe
Aug 1, 2012·Acta Cytologica·Massimo BongiovanniZubair W Baloch
Feb 23, 2013·Nature Reviews. Cancer·Mingzhao Xing
Mar 30, 2013·The Journal of Clinical Endocrinology and Metabolism·Nikhil GuptaSteven P Hodak
Jun 15, 2013·Endocrine-related Cancer·Xiaoli LiuMingzhao Xing
Aug 5, 2014·Cell Reports·Nicolò RigamontiMichele De Palma
Nov 25, 2014·Cell·UNKNOWN Cancer Genome Atlas Research Network
Feb 12, 2015·The New England Journal of Medicine·Martin SchlumbergerSteven I Sherman
Aug 9, 2015·BMC Medicine·Marco MediciErik K Alexander
Jan 7, 2016·Endocrine-related Cancer·Rengyun Liu, Mingzhao Xing
Feb 16, 2016·The Journal of Clinical Investigation·Iñigo LandaJames A Fagin
Aug 16, 2017·The Journal of Surgical Research·Zviadi AburjaniaDawn M Elfenbein
Oct 5, 2017·Cancer Control : Journal of the Moffitt Cancer Center·Pablo Valderrabano, Bryan McIver
Nov 3, 2017·Cytopathology : Official Journal of the British Society for Clinical Cytology·M Decaussin-PetrucciJ Lopez
Jun 1, 2018·Thyroid : Official Journal of the American Thyroid Association·Pablo ValderrabanoBryan McIver

❮ Previous
Next ❯

Citations

Aug 11, 2021·Surgical Pathology Clinics·Juan C Hernandez-Prera

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
hormone replacement therapy
nucleic acid extraction
light microscopy
biopsies

Software Mentioned

miRInform
NanoString nSolver Analysis Software
NanoString nSolver Analysis

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

The New England Journal of Medicine
Marc Pusztaszeri
The New England Journal of Medicine
José M Domínguez
The New England Journal of Medicine
Sam Wiseman, Blair Walker
Gland Surgery
Samira Mercedes Sadowski, Frédéric Triponez
© 2021 Meta ULC. All rights reserved