The lung is an important organ for air breathing in tetrapods and originated well before the terrestrialization of vertebrates. Therefore, to better understand lung evolution, we investigated lung development in the extant basal actinopterygian fish Senegal bichir (Polypterus senegalus). First, we histologically confirmed that lung development in this species is very similar to that of tetrapods. We also found that the mesenchymal expression patterns of three genes that are known to play important roles in early lung development in tetrapods (Fgf10, Tbx4, and Tbx5) were quite similar to those of tetrapods. Moreover, we found a Tbx4 core lung mesenchyme-specific enhancer (C-LME) in the genomes of bichir and coelacanth (Latimeria chalumnae) and experimentally confirmed that these were functional in tetrapods. These findings provide the first molecular evidence that the developmental program for lung was already established in the common ancestor of actinopterygians and sarcopterygians.
Characterization of a new subfamily of winged-helix/forkhead (Fox) genes that are expressed in the lung and act as transcriptional repressors
Basal actinopterygian relationships: a mitogenomic perspective on the phylogeny of the "ancient fish"
T-box gene products are required for mesenchymal induction of epithelial branching in the embryonic mouse lung
Mitogenomic evolution and interrelationships of the Cypriniformes (Actinopterygii: Ostariophysi): the first evidence toward resolution of higher-level relationships of the world's largest freshwater fish clade based on 59 whole mitogenome sequences
Wnt7b stimulates embryonic lung growth by coordinately increasing the replication of epithelium and mesenchyme
Dual hindlimb control elements in the Tbx4 gene and region-specific control of bone size in vertebrate limbs
The genus Polypterus (bichirs): a fish group diverged at the stem of ray-finned fishes (Actinopterygii)
Hox clusters of the bichir (Actinopterygii, Polypterus senegalus) highlight unique patterns of sequence evolution in gnathostome phylogeny
Comparative transcriptome analyses indicate molecular homology of zebrafish swimbladder and mammalian lung
Scales and dermal skeletal histology of an early bony fish Psarolepis romeri and their bearing on the evolution of rhombic scales and hard tissues
Boom and bust: ancient and recent diversification in bichirs (Polypteridae: Actinopterygii), a relictual lineage of ray-finned fishes
The Asian arowana (Scleropages formosus) genome provides new insights into the evolution of an early lineage of teleosts
The Oldest Actinopterygian Highlights the Cryptic Early History of the Hyperdiverse Ray-Finned Fishes
Evolution of Shh endoderm enhancers during morphological transition from ventral lungs to dorsal gas bladder
Dorsoventral inversion of the air-filled organ (lungs, gas bladder) in vertebrates: RNAsequencing of laser capture microdissected embryonic tissue.
Recent advances in our understanding of the mechanisms of late lung development and bronchopulmonary dysplasia
TBX4 is involved in the super-enhancer-driven transcriptional programs underlying features specific to lung fibroblasts
Changes in Nkx2.1, Sox2, Bmp4, and Bmp16 expression underlying the lung-to-gas bladder evolutionary transition in ray-finned fishes.
Does the bowfin gas bladder represent an intermediate stage during the lung-to-gas bladder evolutionary transition?
CREs: Gene & Cell Therapy
Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.