Molecular diagnosis and phylogenetic analysis of human papillomavirus type-16 from suspected patients in Pakistan

Infectious Agents and Cancer
Abdullah AbdullahMuhammad Zeeshan Hyder

Abstract

Human Papillomavirus (HPV) is well known pathogen that can cause benign and malignant tumors in humans, yet there is very little information regarding HPV types prevalent in Pakistan. A total of 92 cervical secretions were collected from suspected married female patients and used for DNA isolation using a novel isolation method. The samples were tested through Polymerase Chain Reaction (PCR) using already reported primers MY09/MY11, GP5/GP6, GP5+/GP6+, CP65/CP70, CP66/CP69 and SPF1/SPF2 and with those developed in this study including HRT1 and HRT2 primer sets for typing HPV types and HACTB primer set for human beta actin gene as internal positive control. Sequencing and phylogenetic analyses were performed for two isolates to determine circulating HPV types. PCR with HRT1 and HRT2 indicated 2 (2.17 %) patients were positive for HPV type- 16 while 1 (1.08 %) with HPV type 18. Sequencing and phylogenetic analysis of isolates confirmed HPV type-16 in genus alpha 9 which have 99 % homology with already reported HPV from Japan and Costa Rica. This is the first report of HPV type-16 genus alpha 9 in Pakistan and the reported assay and sequence data will serve as valuable tools in further epidemiological studies for HPV surveillance ...Continue Reading

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Citations

Aug 31, 2016·Journal of Cancer Research and Clinical Oncology·Naureen Ehsan IlahiSheeba Murad

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Datasets Mentioned

BETA
JX073135
KU212390

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Blast
MAFFT
MEGA
Molecular Evolutionary Genetics Analysis Program ( MEGA )

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