Molecular diagnosis and species identification of mucosal leishmaniasis in Iran and correlation with cytological findings

Acta Cytologica
Sadegh ShirianYahya Daneshbod

Abstract

Mucosal leishmaniasis (ML) is a rare destructive disease that mainly affects the mucous membranes of the mouth and nose. The etiologic agent(s) of ML are not well known in the Middle East. Cytologic smears of ML from the mucosal lesions of 7 patients were prepared by scraping. In 2 patients with nasal lesions, exfoliative cytology was made by washing the nasal cavity. The smears were both air dried and fixed in alcohol and stained. Scrapings from the same smears were then tested for leishmanial DNA by nested PCR. This study characterized 9 isolates of ML, with 7 cases identified as Leishmania major and 2 as Leishmania tropica. While 6 patients were found to be positive by the cytology technique, the nested PCR was positive for all of these samples. Presence of granuloma and multinucleated giant cells in the negative smears of the patients who showed clinical manifestation of ML was an important clue for diagnosis of this disease. The PCR-based method not only appears to be a precise diagnostic approach in the identification of suspected cases of ML but is also efficient in determining the species of the parasite. L. major and L. tropica can lead to ML, but they result in different cytologic features.

Citations

Sep 14, 2012·Journal of Clinical Microbiology·Sadegh ShirianYahya Daneshbod
Jul 23, 2013·PLoS Neglected Tropical Diseases·Yahya SohrabiMarie Lipoldová
Jan 31, 2014·Archives of Pathology & Laboratory Medicine·Sadegh ShirianYahya Daneshbod
Sep 27, 2012·Parasite Immunology·S Nylén, L Eidsmo
Jun 10, 2014·Journal of the European Academy of Dermatology and Venereology : JEADV·H Hammami-GhorbelK Aoun
Jun 23, 2015·Cell & Bioscience·Milena de Paiva-CavalcantiValéria Rêgo Alves Pereira
Jul 31, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ahmad OryanYahya Daneshbod
Jun 14, 2014·Diagnostic Microbiology and Infectious Disease·Ciro M GomesRaimunda N R Sampaio
Mar 28, 2021·Immunology Letters·Maryam AkbariGholamreza Hatam

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