PMID: 11901858Mar 21, 2002Paper

Molecular diagnosis of animal diseases: some experiences over the past decade

Expert Review of Molecular Diagnostics
S Belák, P Thorén

Abstract

The experiences of a veterinary research and diagnostic laboratory are summarized on the development and application of the PCR to diagnose a wide range of viral diseases in animals. The group started the routine diagnostic application of the PCR as early as 1988 and today a total of 35 nested PCR assays are in routine use for the detection of 15 DNA and 20 RNA viruses. Special tools and laboratory practice were applied to avoid false-positive results, while false-negatives are avoided by internal controls (mimics). At present, the classical nested PCR methods are being replaced by real-time TaqMan and molecular beacon assays and the multiplex real-time PCR detection of viruses is also under development. By direct sequencing of the PCR products, phylogeny studies are performed and molecular epizootiology results are provided for rapid and exact identification of virus variants. Molecular epizootiology also contributes to trace the routes of virus spreading on large geographic areas. Recently, large efforts have been made to follow the recommendations of Office International des Epizooties for the standardization and international harmonization of the molecular diagnostic assays.

References

Aug 1, 1996·Virus Research·S VilcekS Belák
Oct 1, 1996·Genome Research·U E GibsonP M Williams
Feb 3, 1998·Nature Biotechnology·S TyagiF R Kramer
Mar 12, 1998·Virus Research·T StadejekS Belák
May 20, 1998·Genome Research·X ChenP Y Kwok
May 5, 2001·Veterinary Microbiology·E W AldousD J Alexander

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Citations

Aug 1, 2008·Transboundary and Emerging Diseases·B Rodriguez-SanchezS Belak
Jan 1, 2004·Irish Veterinary Journal·Jane A Irwin, Alan W Baird
Sep 30, 2009·PLoS Neglected Tropical Diseases·Anthony R FooksThomas Müller
May 14, 2009·Expert Review of Molecular Diagnostics·Sándor BelákGerrit Viljoen
Sep 11, 2008·Research in Veterinary Science·J Fernández-PineroJ M Sánchez-Vizcaíno
Mar 23, 2007·Journal of Virological Methods·Jonas Johansson WensmanMikael Berg
Mar 24, 2005·Acta Veterinaria Hungarica·S Belák
Jun 9, 2007·Acta Veterinaria Hungarica·L SámiI Kiss
Oct 30, 2007·Journal of Virological Methods·Jovita FernándezJosé Manuel Sánchez-Vizcaíno
Jan 12, 2013·Journal of Virological Methods·Sandra Cuevas-RomeroMikael Berg
Sep 10, 2014·Journal of Virological Methods·Kerstin WernikeBernd Hoffmann
May 29, 2016·Transboundary and Emerging Diseases·K WernikeB Hoffmann
Nov 21, 2015·Infection Ecology & Epidemiology·Julieta Sandra Cuevas-RomeroMikael Berg
Jan 18, 2006·Veterinary Parasitology·Franz J Conraths, Gereon Schares
Sep 29, 2004·Journal of Biomolecular Screening·Janice L HuffEric Henderson
Nov 24, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Donna Marie West, Jason Sawyer
Mar 6, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Zhengchun LuUdeni B R Balasuriya
Jul 19, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kerstin WernikeBernd Hoffmann
Nov 25, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M StokstadT Løken
Dec 9, 2021·Journal of Basic Microbiology·Safia ArbabInam Ul Hassan

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