Molecular diagnosis of Arcobacter and Campylobacter in diarrhoeal samples among Portuguese patients

Diagnostic Microbiology and Infectious Disease
Susana FerreiraMónica Oleastro

Abstract

The present study was conducted to investigate the prevalence and diversity of Arcobacter and Campylobacter spp. in 298 stool samples of patients with diarrhoea, collected from 22 Portuguese hospitals, between September and November 2012. Detection of Arcobacter and Campylobacter spp. was performed using molecular-based detection techniques, such as real-time fluorescence resonance energy transfer PCR, species-specific PCR, and sequencing of amplified PCR products. Overall, 1.3% of the samples were positive for Arcobacter butzleri and 0.3% for Arcobacter cryaerophilus. Campylobacter spp. were found in 31.9% of diarrhoeic faeces. Campylobacter jejuni and Campylobacter concisus were the most prevalent species (13.7% and 8.0%, respectively). The prevalence of Arcobacter and Campylobacter spp. was significantly different between children and adults (39.7% versus 22.8%, P = 0.003). We underline the high prevalence of these pathogens in diarrhoeal samples among Portuguese patients, with particular relevance in the paediatric age group.

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Citations

Mar 26, 2015·Critical Reviews in Microbiology·Susana FerreiraFernanda C Domingues
Dec 25, 2015·Journal of Medical Microbiology·Alexander P UnderwoodHazel M Mitchell
Sep 15, 2014·Microbial Pathogenesis·Susana FerreiraFernanda C Domingues
Aug 19, 2014·International Journal of Antimicrobial Agents·Andreia DuarteMónica Oleastro
Jun 5, 2016·Veterinary Microbiology·Mehmet Nuri AçikBurhan Çetinkaya
May 15, 2016·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·Ingrid HänelHeinrich Neubauer
Jan 18, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Susana FerreiraFernanda C Domingues
Jun 13, 2015·Clinical Microbiology Reviews·Nadeem O KaakoushSi Ming Man
Mar 17, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Alper TekeliIstar Dolapci
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Daniele ChieffiVincenzina Fusco
Jun 22, 2021·Antimicrobial Agents and Chemotherapy·Susana FerreiraMónica Oleastro

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