Molecular diagnosis of avian nephritis: preliminary report

Acta Veterinaria Hungarica
Mira MándokiM Rusvai

Abstract

Kidney samples from chickens diagnosed with acute nephritis and gout were subjected to histological and electron microscopic examination. The investigations revealed cytoplasmic inclusion bodies in the tubular epithelial cells containing round virions of about 30 nm in diameter. Since avian nephritis virus (ANV) is known as a potential causative agent of the so-called baby chick nephropathy, an RT-PCR assay was developed for the molecular detection of ANV-specific nucleic acid in the specimen. The specificity of the assay was confirmed by direct sequencing of the amplicon obtained in the reaction. The nucleotide sequence of the PCR product showed 92% identity with the reference ANV sequence deposited in the GenBank database. After having been validated on some other suspicious cases of avian nephritis, the PCR method described in this study can be a potential tool for routine diagnostic examination of samples submitted from cases of gout and nephropathy in chickens.

References

Jan 7, 1998·Bone Marrow Transplantation·B J BolwellB Pohlman
Oct 25, 2002·Avian Pathology : Journal of the W.V.P.A·Matthew D Koci, Stacey Schultz-Cherry
Jun 30, 2004·Avian Pathology : Journal of the W.V.P.A·William Baxendale, Teshome Mebatsion
Jul 1, 1981·Avian Pathology : Journal of the W.V.P.A·W G Siller

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Citations

Jun 7, 2006·Avian Pathology : Journal of the W.V.P.A·Míra MándokiMiklós Rusvai
Jun 15, 2010·Avian Pathology : Journal of the W.V.P.A·D ToddB M Adair
Jun 30, 2011·Avian Pathology : Journal of the W.V.P.A·D ToddM D Welsh
Nov 5, 2019·Transboundary and Emerging Diseases·Qinxi ChenQingmei Xie
Sep 7, 2018·Emerging Microbes & Infections·Xinyu ZhangJianqiang Ye

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