Molecular diagnosis of cutaneous leishmaniasis and species identification: analysis of 122 biopsies with varied parasite index

Journal of Cutaneous Pathology
Lamis YehiaIbrahim Khalifeh

Abstract

Cutaneous leishmaniasis is endemic in the Middle East and North Africa. Confirming the diagnosis histologically depends on amastigote identification, which varies significantly depending on the inoculum, strain type, host response and disease stage. Accurate histological diagnosis is mandatory for appropriate therapy. Skin biopsies from 122 patients from Lebanon, Syria and Saudi Arabia with clinical diagnosis of untreated leishmaniasis were reviewed and clinical data extracted. Cases were classified according to the modified Ridley's parasitic index. DNA was extracted from formalin-fixed paraffin-embedded blocks. Polymerase chain reaction (PCR) was performed using Leishmania-specific ribosomal internal transcribed spacer 1 (ITS1-PCR). Nested ITS1-PCR was performed on cases negative for conventional ITS1-PCR. ITS1-PCR amplicons were digested with HaeIII for subsequent restriction fragment length polymorphism (RFLP) subspeciation. Of 122 cases, 54 (44.3%) showed a parasitic index of 0-1+ (no unequivocal amastigotes). ITS1-PCR (conventional and nested) was positive for all cases as compared with negative control tissue. RFLP identified Leishmania tropica in all cases. Patients with clinically suspected leishmaniasis, whose skin bi...Continue Reading

References

Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Oct 1, 1990·Experimental Parasitology·M R RodgersD F Wirth
Jan 1, 1989·Parasitology·D C Barker
Jan 1, 1988·The Journal of Experimental Medicine·D K ShibataW J Martin
Jul 16, 1985·Biochemical and Biophysical Research Communications·S E GoelzB Vogelstein
Sep 1, 1983·The Journal of Pathology·D S Ridley, M J Ridley
May 1, 1995·Transactions of the Royal Society of Tropical Medicine and Hygiene·T LaskayD Frommel
Jul 1, 1995·Molecular and Biochemical Parasitology·E CupolilloS M Beverley
Feb 1, 1996·Archives of Dermatology·A Z MomeniJ Tada
Oct 8, 1997·Journal of Clinical Microbiology·O F OsmanP A Kager
Oct 8, 1997·Journal of Clinical Microbiology·P MinodierH Dumon
Jun 29, 1999·Clinics in Dermatology·S M SalmanA G Kibbi
Jul 30, 2002·Dermatology : International Journal for Clinical and Investigative Dermatology·Akbar SafaeiMohammad Vasei
Aug 9, 2002·Tropical Medicine & International Health : TM & IH·Henk D F H Schallig, Linda Oskam
Feb 8, 2003·Journal of Clinical Microbiology·Tiago M CastilhoLucile M Floeter-Winter
May 8, 2003·Current Opinion in Infectious Diseases·Francisco Vega-López
Sep 12, 2003·Diagnostic Microbiology and Infectious Disease·Gabriele SchönianCharles L Jaffe
Apr 19, 2005·Expert Review of Molecular Diagnostics·Sarman SinghRamu Sivakumar
Apr 7, 2006·Journal of Clinical Microbiology·Esther BensoussanCharles L Jaffe
Aug 5, 2006·Travel Medicine and Infectious Disease·J J ScarisbrickF Vega-López
Aug 3, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Gregory SpanakosNicholas C Vakalis
Feb 12, 2008·Trends in Parasitology·Gabriele SchönianJean-Claude Dujardin
Nov 6, 2009·Dermatologic Therapy·Consuelo V David, Noah Craft
Dec 2, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andrea K BoggildDonald E Low
Apr 10, 2010·Expert Review of Anti-infective Therapy·Hiro Goto, José Angelo Lauletta Lindoso
Jun 22, 2010·Tropical Medicine & International Health : TM & IH·Kifaya AzmiZiad Abdeen
Sep 14, 2010·Clinical and Experimental Dermatology·M Ameen

❮ Previous
Next ❯

Citations

Oct 4, 2014·Emerging Infectious Diseases·Maya SaroufimIbrahim Khalifeh
Nov 19, 2015·Journal of the American Academy of Dermatology·Angel Fernandez-Flores, José Luis Rodriguez-Peralto
Nov 24, 2012·Seminars in Cutaneous Medicine and Surgery·Brian L Swick
Oct 3, 2014·Parasites & Vectors·Atitaya HitakarunSaovanee Leelayoova
Apr 21, 2012·Journal of Cutaneous Pathology·Timothy H McCalmont
Mar 17, 2016·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Gabriel DunyaIbrahim Khalifeh
Jun 23, 2015·Cell & Bioscience·Milena de Paiva-CavalcantiValéria Rêgo Alves Pereira
Oct 24, 2014·PLoS Neglected Tropical Diseases·Jessica AounIbrahim Khalifeh
Jun 3, 2016·Journal of Cutaneous Pathology·Elias ZgheibIbrahim Khalifeh
Feb 11, 2016·PLoS Neglected Tropical Diseases·Elie AlamIbrahim Khalifeh
Sep 4, 2014·Anais Brasileiros De Dermatologia·Ciro Martins GomesRaimunda Nonata Ribeiro Sampaio
Jan 7, 2015·The American Journal of Tropical Medicine and Hygiene·Mario SaabIbrahim Khalifeh
Apr 29, 2018·Journal of the European Academy of Dermatology and Venereology : JEADV·G Merino-EspinosaJ Martín-Sánchez
Jan 6, 2018·Journal of Global Infectious Diseases·Miskelyemen Abdelatti ElmekkiAli H Alharbi
Nov 17, 2016·Journal of Clinical Microbiology·Marcos E de AlmeidaAlexandre J da Silva
Apr 11, 2019·International Journal of Dermatology·Maelle SalibaIbrahim Khalifeh
Jul 17, 2019·BMC Infectious Diseases·Bhagya DeepachandiYamuna Siriwardana
Aug 5, 2017·Journal of the European Academy of Dermatology and Venereology : JEADV·G Merino-EspinosaJ Martín-Sánchez
Jun 23, 2015·International Journal of Dermatology·Ana M MontalvoVirginia Capó de Paz
Feb 23, 2017·The Journal of International Medical Research·Fahriye EkşiMustafa Sağlam
Oct 15, 2020·Experimental Parasitology·Rayana Carla Silva de MoraisMilena de Paiva Cavalcanti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.