Molecular diagnosis of renal-allograft rejection: correlation with histopathologic evaluation and antirejection-therapy resistance

Transplantation
D DesvauxSabine Le Gouvello

Abstract

Because histopathologic criteria cannot always predict the pathogenesis and response to curative antirejection therapy, new hope derives from the molecular analysis of intragraft immunologic markers. We studied whether the cutoff of intragraft expression level of T-cell activation markers may define subgroups of acute rejection differing either in type of rejection or clinical outcome. Forty-three human renal-allograft biopsies were quantified for mRNA expression of granzyme B, Fas ligand, interferon (IFN)gamma, interleukin (IL)-4, and IL-6 with a reverse-transcriptase real-time quantitative polymerase chain reaction (RT-PCR) method. Expression levels were correlated with the histopathologic rejection type according to the Banff 1997 classification criteria, and with the sensitivity to the antirejection immunosuppressive therapy, by means of receiver operating-characteristic (ROC) curves. Granzyme B and Fas ligand mRNA expression up-regulation correlated with all allograft rejection types (P<0.01 for all). Moreover, granzyme B showed the highest sensitivity (90%) and specificity (78%) for the potential detection of histologic borderline changes that will require immunosuppressive therapy (area under the curve [AUC]=0.856, P<0.0...Continue Reading

References

Mar 1, 1991·Transplantation·C VandenbroeckeM G Tovey
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·K KarmannJ S Pober
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·J StrehlauT B Strom
Nov 14, 1997·The Journal of Experimental Medicine·D HamannR A van Lier
Feb 13, 1999·Kidney International·L C RacusenY Yamaguchi
Aug 6, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·F I MacdonaldI S Roberts
Aug 14, 1999·Journal of the American Society of Nephrology : JASN·P NickersonD Rush
Oct 9, 1999·Transplantation·D A CharneyL C Racusen
Jun 23, 2000·Journal of the American Society of Nephrology : JASN·Corina LorzJesús Egido
Jan 6, 2001·The American Journal of Pathology·P F HalloranN M Kneteman
May 23, 2002·Transplantation·Alain Le MoineDaniel Abramowicz
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arno MüllbacherMarkus M Simon
Mar 20, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Dominique DesvauxChristophe Baron

❮ Previous
Next ❯

Citations

Jan 4, 2012·International Urology and Nephrology·Amin RoshdyAmal M El-Shehaby
Oct 6, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Michael EikmansJan A Bruijn
Jun 7, 2013·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ondrej ViklickyIrena Brabcova
Feb 17, 2006·Current Opinion in Nephrology and Hypertension·Holger SchmidMatthias Kretzler
Sep 27, 2012·Artificial Organs·Lining JiaYan Ou
Aug 1, 2008·Journal of the American Society of Nephrology : JASN·Hicham MansourPhilippe Grimbert
Sep 1, 2014·Surgical Pathology Clinics·Michael Mengel
Mar 4, 2006·Expert Review of Molecular Diagnostics·Qiuheng Zhang, Elaine F Reed
Mar 17, 2011·Expert Review of Molecular Diagnostics·Valeria R MasDaniel G Maluf
Dec 1, 2008·Expert Opinion on Medical Diagnostics·Michael EikmansFrans Hj Claas
Jan 25, 2008·Kidney International·E C Aquino-DiasR C Manfro
Jan 15, 2009·Transplant Immunology·J CarstensH E Hansen
Jun 5, 2007·Heart Failure Clinics·Richard N Pierson
Mar 3, 2007·Transplant Immunology·Viviane C PoncianoAlvaro Pacheco-Silva
May 23, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K L Womer, B Kaplan
Jul 11, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K DahanP Grimbert
Aug 8, 2015·BMC Nephrology·Benjamin Adam, Michael Mengel
Feb 1, 2015·Transplantation Reviews·Eva GirmanovaOndrej Viklicky
Apr 26, 2016·Transplant Immunology·Fatina I FadelEman H Thabet
Mar 18, 2016·Transplantation·Rajil MehtaSundaram Hariharan
Aug 3, 2016·Transplant Immunology·Niels V RekersMichael Eikmans
Nov 28, 2007·Annual Review of Pathology·Lynn D CornellRobert B Colvin
Apr 13, 2010·Expert Review of Clinical Immunology·Fadi IssaKathryn J Wood
Jun 22, 2011·Kidney & Blood Pressure Research·Petra Hribova, Ondrej Viklicky
Mar 28, 2020·International Journal of Molecular Sciences·Francesc MoresoDaniel Serón
Mar 10, 2020·Transplantation·Malou L H SnijdersMarian C Clahsen-van Groningen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
J StrehlauTerry B Strom
American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Tania SimonCaner Süsal
Clinical Transplantation
Esther Cristina Aquino DiasRoberto Ceratti Manfro
© 2021 Meta ULC. All rights reserved