Molecular diagnostic assays for COVID-19: an overview.

Critical Reviews in Clinical Laboratory Sciences
Parham HabibzadehMohammad Ali Faghihi


The coronavirus disease 2019 (COVID-19) pandemic has highlighted the cardinal importance of rapid and accurate diagnostic assays. Since the early days of the outbreak, researchers with different scientific backgrounds across the globe have tried to fulfill the urgent need for such assays, with many assays having been approved and with others still undergoing clinical validation. Molecular diagnostic assays are a major group of tests used to diagnose COVID-19. Currently, the detection of SARS-CoV-2 RNA by reverse transcription polymerase chain reaction (RT-PCR) is the most widely used method. Other diagnostic molecular methods, including CRISPR-based assays, isothermal nucleic acid amplification methods, digital PCR, microarray assays, and next generation sequencing (NGS), are promising alternatives. In this review, we summarize the technical and clinical applications of the different COVID-19 molecular diagnostic assays and suggest directions for the implementation of such technologies in future infectious disease outbreaks.


Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Nov 16, 2001·Biochemical and Biophysical Research Communications·Y MoriT Notomi
Mar 5, 2004·Chembiochem : a European Journal of Chemical Biology·J Manuel PerezRalph Weissleder
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fredrik DahlMats Nilsson
May 7, 2004·Applied and Environmental Microbiology·Gary J VoraJoanne D Andreadis
May 14, 2008·BioTechniques·Heather D VanGuilderWillard M Freeman
Jun 20, 2008·Journal of Clinical Microbiology·Noah G HoffmanKeith R Jerome
Nov 1, 2011·Analytical Chemistry·Benjamin J HindsonBill W Colston
Sep 3, 2013·Nature Methods·Christopher M HindsonMuneesh Tewari
Sep 5, 2013·Clinical Chemistry·Tanis C DingleKeith R Jerome
Dec 3, 2014·Virus Research·Marion SevajolIsabelle Imbert
Mar 5, 2015·PloS One·John F ReganSteven A McCarroll
Apr 15, 2016·Biomolecular Detection and Quantification·David SvecMikael Kubista
Sep 28, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Frank Hille, Emmanuelle Charpentier
Dec 19, 2016·Biomolecular Detection and Quantification·Alexandra S WhaleSvilen Tzonev
Mar 17, 2017·Journal of Clinical Microbiology·Jane Kuypers, Keith R Jerome
Apr 15, 2017·Science·Jonathan S GootenbergFeng Zhang
May 26, 2017·Viruses·Stephen HayesDouwe van Sinderen
Sep 13, 2017·Nature Biotechnology·Christopher QuinceNicola Segata
Apr 21, 2018·Sensors·Phenix-Lan QuanEric Brouzes
Apr 28, 2018·Science·Cameron MyhrvoldPardis C Sabeti
May 10, 2018·Frontiers in Microbiology·Sam NooijMarion P G Koopmans
Nov 18, 2018·MSystems·Benjamin HillmannDan Knights
Mar 29, 2019·Nature Reviews. Genetics·Charles Y Chiu, Steven A Miller
May 31, 2019·Nature Reviews. Molecular Cell Biology·Adrian Pickar-Oliver, Charles A Gersbach
Sep 25, 2019·Nature Protocols·Max J KellnerFeng Zhang
Sep 26, 2019·Frontiers in Neurology·Parham HabibzadehMohammad Ali Faghihi
Nov 11, 2019·Clinical Chemistry·Stephen J Salipante, Keith R Jerome
Jan 30, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Victor M CormanChristian Drosten
Feb 6, 2020·The International Journal of Occupational and Environmental Medicine·Parham Habibzadeh, Emily K Stoneman
Feb 8, 2020·Clinical Chemistry·Daniel K W ChuLeo L M Poon
Feb 23, 2020·Viruses·Daniel KiselevKamil Khafizov
Feb 23, 2020·JAMA : the Journal of the American Medical Association·Yan BaiMeiyun Wang

❮ Previous
Next ❯


Mar 23, 2021·Expert Review of Clinical Immunology·Rohan AmeratungaAnna E S Brooks
Aug 8, 2021·Diagnostics·Melissa R GitmanEmilia M Sordillo
Aug 19, 2021·BMC Research Notes·Muhammad Zain MushtaqZahra Hasan

❮ Previous
Next ❯

Datasets Mentioned


Methods Mentioned

nucleic acid amplification

Software Mentioned


Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.