Molecular differences between stromal cell populations from deciduous and permanent human teeth

Stem Cell Research & Therapy
Nina KaukuaKaj Fried

Abstract

Deciduous and permanent human teeth represent an excellent model system to study aging of stromal populations. Aging is tightly connected to self-renewal and proliferation and thus, mapping potential molecular differences in these characteristics between populations constitutes an important task. Using specifically designed microarray panels, Real-Time Quantitative Polymerase Chain Reaction (RT q-PCR), Western blot, immunohistochemistry and siRNA-mediated knock down experiments, we have detected a number of molecules that were differentially expressed in dental pulp from deciduous and permanent teeth extracted from young children and adults, respectively. Among the differentially regulated genes, high-mobility group AT-hook 2 (HMGA2), a stem cell-associated marker, stood out as a remarkable example with a robust expression in deciduous pulp cells. siRNA-mediated knock down of HMGA2 expression in cultured deciduous pulp cells caused a down-regulated expression of the pluripotency marker NANOG. This finding indicates that HMGA2 is a pulpal stem cell regulatory factor. In addition to this, we discovered that several proliferation-related genes, including CDC2A and CDK4, were up-regulated in deciduous pulp cells, while matrix genes...Continue Reading

References

Dec 1, 1974·Journal of Pediatric Surgery·C M Illingworth
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·Stan GronthosS Shi
Jul 31, 2002·Journal of Dental Research·Stan GronthosS Shi
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masako MiuraS Shi
Feb 20, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexander Y MaslovSteven C Pruitt
Sep 24, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Emeka EnwereSamuel Weiss
Aug 25, 2007·Nature Reviews. Molecular Cell Biology·Norman E Sharpless, Ronald A DePinho
Apr 2, 2008·Clinical and Investigative Medicine. Médecine Clinique Et Experimentale·Stefano TetePio Conti
Dec 17, 2008·Cell·Scott M Hammond, Norman E Sharpless
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Sep 2, 2009·Journal of Endodontics·Michelangelo TranasiStefano Tetè
Feb 4, 2010·Biochimica Et Biophysica Acta·Hena R AsharKiran Chada
Aug 24, 2010·Journal of Endodontics·Vijayendran GovindasamyNoor Hayaty Abu Kasim
May 19, 2011·Journal of Dental Research·T SuzukiJ J Mao
Aug 31, 2011·Methods in Molecular Biology·Daniele MericoGary D Bader
Jul 28, 2012·Dental Clinics of North America·Anthony J SmithPaul R Cooper
Jan 21, 2014·Current Topics in Developmental Biology·Yunjoon Jung, Andrew S Brack

Citations

Jun 7, 2019·Stem Cells and Development·Lewis WinningFionnuala T Lundy
Jul 15, 2020·Biology·Shinichiro YoshidaHidefumi Maeda
May 18, 2018·Frontiers in Bioengineering and Biotechnology·Mona K Marei, Rania M El Backly
Sep 22, 2020·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Mohammad AlansaryDawn Coates

Related Concepts

NANOG protein, human
CDK4 protein, human
CDC2 protein, human
Nanog Homeobox Protein
Senility
Dental Pulp
Deciduous Tooth
Receptor Down-Regulation
Stromal Cells
Homeodomain Proteins

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