Molecular dissection of two homoeologous wheat genes encoding RING H2-type E3 ligases: TaSIRFP-3A and TaSIRFP-3B.

Planta
Yong Chan Park, Cheol Seong Jang

Abstract

Two homoeologous wheat genes, TaSIRFP-3A and TaSIRFP-3B, encode the RING-HC-type E3 ligases that play an inhibitory role in sucrose metabolism in response to cold stress. In higher plants, the attachment of ubiquitin (Ub) and the subsequent recognition and degradation by the 26S proteasome affects a variety of cellular functions that are essential for survival. Here, we characterized the two homoeologous wheat genes encoding the really interesting new gene (RING) HC-type E3 ligases: TaSIRFP-3A and TaSIRFP-3B (Triticum aestivum SINA domain including RING finger protein 1 and 2), which regulate target proteins via the Ub/26S proteasome system. The TaSIRFP-3A gene was highly expressed under cold stress. In contrast, its homoeologous gene, TaSIRFP-3B, showed only a slight increase in expression levels in shoots. Despite these differences, both the proteins exhibited E3 ligase activity with the cytosol- and nucleus-targeted localization, demonstrating their conserved molecular function. Heterogeneous overexpression of TaSIRFP-3A or TaSIRFP-3B in Arabidopsis showed delayed plant growth causing a reduction in sucrose synthase enzymatic activity and photosynthetic sucrose synthesis, by regulating sucrose synthase proteins. TaSIRFP-3A- ...Continue Reading

References

Feb 8, 1991·Cell·P S FreemontJ Trowsdale
Jun 1, 1996·Current Opinion in Structural Biology·K L Borden, P S Freemont
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 24, 2004·Journal of Experimental Botany·Sébastien BaudChristine Rochat
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Graham Noctor, Christine H. Foyer
Sep 21, 2004·Annual Review of Plant Biology·Jan Smalle, Richard D Vierstra
Dec 8, 2004·The Plant Cell·Jennifer MoonMark Estelle
Jan 13, 2005·Plant Physiology·Sophia L StoneJudy Callis
Aug 11, 2006·The Plant Journal : for Cell and Molecular Biology·A BottleyR M D Koebner
Dec 21, 2006·Plant Biotechnology Journal·Heather D ColemanShawn D Mansfield
Jan 30, 2007·The Plant Journal : for Cell and Molecular Biology·Zuzanna BieniawskaAlison M Smith
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Rainer WaadtJörg Kudla
Dec 17, 2008·Methods in Molecular Biology·Claus SchwechheimerEsther M N Dohmann
May 9, 2009·Nature Reviews. Molecular Cell Biology·Richard D Vierstra
Jun 6, 2009·Annual Review of Biochemistry·Raymond J Deshaies, Claudio A P Joazeiro
Apr 23, 2010·The Plant Journal : for Cell and Molecular Biology·Aaron Santner, Mark Estelle
Feb 25, 2011·Molecules and Cells·Jae-Hoon Lee, Woo Taek Kim
Jun 2, 2011·Molecular Genetics and Genomics : MGG·Yanze LiChengchao Zheng
Feb 28, 2014·Database : the Journal of Biological Databases and Curation·Janos X BinderLars Juhl Jensen
Mar 5, 2014·Frontiers in Plant Science·Vincent Duplan, Susana Rivas
Jul 9, 2014·Frontiers in Plant Science·Alexandra S Tauzin, Thierry Giardina
Jul 19, 2014·Science·Kellye EversoleJane Rogers
May 31, 2015·Plant Science : an International Journal of Experimental Plant Biology·Peter BuchnerSabine Tausz-Posch
Dec 15, 2015·Biotechnology Advances·Katharina SchmölzerBernd Nidetzky
Feb 26, 2019·Frontiers in Plant Science·Ofer Stein, David Granot

❮ Previous
Next ❯

Methods Mentioned

BETA
ubiquitination
two-hybrid
pull-down
transgenic
Y2H
immunoprecipitation

Software Mentioned

Ensembl
ImageJ
Excel
Ensemble
LSM

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.

Related Papers

Neoplasia : an International Journal for Oncology Research
Yi Sun
World Journal of Clinical Oncology
Barbara C SnoekGodefridus J Peters
Current Opinion in Plant Biology
Claus Schwechheimer, Luz Irina A Calderon Villalobos
© 2021 Meta ULC. All rights reserved