Molecular dissection of venom from Chinese scorpion Mesobuthus martensii: identification and characterization of four novel disulfide-bridged venom peptides

Peptides
Xian-Chun ZengWenxin Li

Abstract

Scorpion venom is composed of a large repertoire of biologically active polypeptides. However, most of these peptides remain to be identified and characterized. In this paper, we report the identification and characterization of four novel disulfide-bridged venom peptides (named BmKBTx, BmKITx, BmKKx1 and BmKKx2, respectively) from the Chinese scorpion, Mesobuthus martensii (also named Buthus martensii Karsch). BmKBTx is composed of 58 amino acid residues and cross-linked by three disulfide bridges. The sequence of BmKBTx shows some similarities to that of the toxin, birtoxin, and its analogs. It is likely that BmKBTx is a beta-toxin active on Na+ channels, which is toxic to either insects or mammals. BmKITx is composed of 71 amino acid residues with four disulfide bridges. It is the longest venom peptide identified from M. martensii so far. BmKITx shows little sequence identity with scorpion alpha-toxins toxic to insects. It is likely that BmKITx is a new type of Na+ -channel specific toxin active on both insects and mammals. BmKKx1 contains 38 amino acid residues cross-linked by three disulfide bridges and shows 84% sequence identity with BmTx3, an inhibitor of A-type K+ channel and HERG currents. BmKKx1 has been classified a...Continue Reading

References

Jan 1, 1980·Annual Review of Pharmacology and Toxicology·W A Catterall
Sep 22, 1999·European Journal of Biochemistry·L D PossaniJ Tytgat
Jan 14, 2001·The Journal of Biological Chemistry·Y V KorolkovaE V Grishin
Nov 21, 2001·Rapid Communications in Mass Spectrometry : RCM·A M PimentaM F Martin-Eauclaire
Aug 2, 2002·The Journal of Biological Chemistry·Yuliya V KorolkovaEugene V Grishin
Nov 28, 2002·The Journal of Biological Chemistry·Jessy DeshaneHarald Sontheimer
Nov 30, 2002·Toxicon : Official Journal of the International Society on Toxinology·Xian-Chun ZengWen-Xin Li
Dec 7, 2002·Toxicon : Official Journal of the International Society on Toxinology·Christian LegrosMarie-France Martin-Eauclaire
Dec 31, 2002·Biochemical and Biophysical Research Communications·Ignacio López-GonzálezAlberto Darszon
Feb 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·Dalia Gordon, Michael Gurevitz
Mar 15, 2003·Biochimica Et Biophysica Acta·Hélène VacherMarie-France Martin-Eauclaire
May 28, 2003·Trends in Pharmacological Sciences·Ricardo C Rodríguez de la VegaLourival D Possani
Jun 5, 2003·Cell Biochemistry and Biophysics·Kenneth M Blumenthal, Anna L Seibert
Sep 19, 2003·European Biophysics Journal : EBJ·Jamie I VandenbergPhilip W Kuchel
Jun 8, 2004·Current Drug Targets. CNS and Neurological Disorders·Thomas BlankJoachim Spiess
Jun 23, 2004·Toxicon : Official Journal of the International Society on Toxinology·Kathleen M GiangiacomoTheodore J Mullmann
Jun 23, 2004·Toxicon : Official Journal of the International Society on Toxinology·Hélène VacherMarie France Martin-Eauclaire
Oct 29, 2004·Nature Reviews. Genetics·Gil Ast
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Elia Diego-GarcíaLourival D Possani
Apr 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Bora InceogluBruce D Hammock
Jun 21, 2005·Biochemical and Biophysical Research Communications·Marie-France Martin-EauclairePierre E Bougis
Jul 23, 2005·IUBMB Life·Xian-Chun ZengRichard Hahin

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Citations

Aug 3, 2012·BMC Genomics·Diego D AlmeidaMatheus de F Fernandes-Pedrosa
Mar 1, 2014·Toxins·Zhijian CaoWenxin Li
May 2, 2014·Journal of Proteomics·Xiaobo XuWenxin Li
Jul 13, 2007·Biochemical and Biophysical Research Communications·Xin MaoWenxin Li
Feb 11, 2011·Journal of Biochemical and Molecular Toxicology·Zongyun ChenWenxin Li
Jul 31, 2013·Toxicon : Official Journal of the International Society on Toxinology·V Quintero-HernándezL D Possani
Jan 5, 2011·Toxicon : Official Journal of the International Society on Toxinology·Song HanXiaohua He
Sep 4, 2013·Toxicon : Official Journal of the International Society on Toxinology·Mohamed A Abdel-RahmanLourival D Possani
Sep 23, 2014·Toxicon : Official Journal of the International Society on Toxinology·Saoussen Mlayah-BellalounaNajet Srairi-Abid
Jan 20, 2011·Amino Acids·Chibuike C UdenigweRotimi E Aluko
Dec 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wanxia ShiXiaoming Chen

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