Molecular diversity of nifH genes from bacteria associated with high arctic dwarf shrubs.

Microbial Ecology
Julie R Deslippe, Keith N Egger

Abstract

Biological nitrogen fixation is the primary source of new N in terrestrial arctic ecosystems and is fundamental to the long-term productivity of arctic plant communities. Still, relatively little is known about the nitrogen-fixing microbes that inhabit the soils of many dominant vegetation types. Our objective was to determine which diazotrophs are associated with three common, woody, perennial plants in an arctic glacial lowland. Dryas integrifolia, Salix arctica, and Cassiope tetragona plants in soil were collected at Alexandra Fiord, Ellesmere Island, Canada. DNA was extracted from soil and root samples and a 383-bp fragment of the nifH gene amplified by the polymerase chain reaction. Cloned genotypes were screened for similarity by restriction fragment length polymorphism (RFLP) analysis. Nine primary RFLP phylotypes were identified and 42 representative genotypes selected for sequencing. Majority of sequences (33) were type I nitrogenases, whereas the remaining sequences belonged to the divergent, homologous, type IV group. Within the type I nitrogenases, nifH genes from posited members of the Firmicutes were most abundant, and occurred in root and soil samples from all three plant species. nifH genes from posited Pseudomo...Continue Reading

References

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Citations

Jun 20, 2008·Microbial Ecology·Laurence DucJosef Zeyer
Mar 21, 2007·Applied and Environmental Microbiology·Daniel H BuckleyTyrrell A Nelson
Aug 16, 2015·Research in Microbiology·Aimeric BlaudA Mark Osborn
Nov 16, 2011·Bioinformatics and Biology Insights·Shaneka S SimmonsHari H P Cohly
Jun 15, 2018·FEMS Microbiology Ecology·Maisie V NashPatricia Sánchez-Baracaldo
Dec 10, 2016·Frontiers in Microbiology·C Ryan PentonJizhong Zhou

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