PMID: 2509661Oct 1, 1989Paper

Molecular dosimetry of 8-MOP + UVA-induced DNA photoadducts in Saccharomyces cerevisiae: correlation of lesions number with genotoxic potential

Journal of Photochemistry and Photobiology. B, Biology
M Bankmann, M Brendel

Abstract

Acid hydrolysis of purified DNA extracted from cells of a haploid repair-proficient (RAD) yeast strain that had been treated with 8-MOP + UVA revealed the existence of two major and one minor thymine photoproduct. At survival levels of the RAD strain between 100% and 1% furanside monoadducts constituted the major DNA lesion, followed by diadducts that, at the lowest survival level, nearly reached 50% of the thymine photoproducts; pyrone-side monoadducts were only detectable at the highest UVA exposure dose applied and clearly constitute a minority photoproduct. The number of induced diadducts was verified by determination of interstrand cross-links via denaturation and renaturation of 8-MOP + UVA-treated DNA from RAD and rad2 yeast strain. 8-MOP + UVA was shown to induce two types of locus-specific mutations: reversion of the lys1-1 ochre allele was between 20- to 50-fold higher than that of the his4-38 frameshift allele. Mutant yield for the lys 1-1 reversion was the same in RAD and excision repair-deficient rad2-20 strains whereas frameshift mutagenesis was about eightfold higher in the rad2-20 background.

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Citations

Feb 1, 1989·Journal of Photochemistry and Photobiology. B, Biology·M Bankmann, M Brendel
Jun 1, 1990·Journal of Photochemistry and Photobiology. B, Biology·J CadetW R Midden
Jun 1, 1990·Journal of Photochemistry and Photobiology. B, Biology·A R Young
Jun 30, 1992·Journal of Photochemistry and Photobiology. B, Biology·D AverbeckE Sage
Dec 4, 2003·Mutation Research·Martin BrendelJoão Antonio Pegas Henriques
Feb 9, 2006·Chemical Reviews·David M NollPaul S Miller
Dec 1, 1989·Photochemistry and Photobiology·D Averbeck
Jul 27, 2010·Environmental and Molecular Mutagenesis·Randy J Legerski
Nov 14, 2006·FEMS Microbiology Reviews·Peter LehoczkýMiroslav Chovanec
Dec 1, 1990·Current Genetics·J A Henriques, M Brendel
Sep 15, 1992·Journal of Photochemistry and Photobiology. B, Biology·J CadetL Voituriez

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