PMID: 1445932Nov 17, 1992Paper

Molecular dynamics of luteinizing hormone receptors on rat luteal cells

Biochimica Et Biophysica Acta
D A RoessB G Barisas

Abstract

To better understand the in situ organization of the luteinizing hormone receptor on rat luteal cells, we have examined the molecular motions of this receptor following binding of ovine luteinizing hormone (oLH) or human chorionic gonadotropin (hCG). Fluorescence photobleaching recovery (FPR) measurements of LH receptor lateral diffusion were performed using tetramethylrhodamine isothiocyanate (TRITC)-derivatized oLH or hCG as a probe. These experiments indicate that TRITC-oLH occupied LH receptors on luteal cells obtained from superovulated female rats have a lateral diffusion coefficient D of (1.7 +/- 0.6).10(-10) cm2s-1 at 27 degrees C with fluorescence recovery after photobleaching of 46 +/- 5%. In similar experiments, binding of TRITC-hCG caused a significant decrease in LH receptor lateral diffusion; fluorescence recovery after photobleaching was less than 20%. To determine whether hCG-occupied receptors might exist in large aggregates, we measured the rotational correlation times (RCT) of hCG and oLH bound to the LH receptor on intact cells using single cell polarized fluorescence depletion (PFD). At 4 degrees C, LH receptors occupied by eosin isothiocyanate (EITC)-hCG exhibited a slower RCT (64 microseconds) than did re...Continue Reading

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Citations

Jun 22, 2011·The Journal of Biological Chemistry·Amber L Wolf-RingwallB George Barisas
Mar 23, 2000·Biochimica Et Biophysica Acta·D A RoessB G Barisas
Mar 2, 1999·Biochemical and Biophysical Research Communications·R D HorvatD A Roess
Aug 2, 2003·Biology of Reproduction·Deborah A Roess, Steven M L Smith
Feb 10, 2010·Personality and Social Psychology Review : an Official Journal of the Society for Personality and Social Psychology, Inc·David A Kenny, Tessa V West

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