Molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds.

Horticulture Research
Chiara FortiAlma Balestrazzi

Abstract

Seed priming, a pre-sowing technique that enhances the antioxidant/DNA repair activities during the pre-germinative metabolism, still retains empirical features. We explore for the first time the molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds in order to identify hallmarks (expression patterns of antioxidant/DNA repair genes combined with free radical profiles) useful to discriminate between high- and low-quality lots. The hydropriming protocol hereby developed anticipated (or even rescued) germination, when applied to lots with variable quality. ROS (reactive oxygen species) raised during hydropriming and dropped after dry-back. Upregulation of antioxidant/DNA repair genes was observed during hydropriming and the subsequent imbibition. Upregulation of SmOGG1 (8-oxoguanine glycosylase/lyase) gene detected in primed seeds at 2 h of imbibition appeared as a promising hallmark. On the basis of these results, the investigation was restricted within the first 2 h of imbibition, to verify whether the molecular landscape was reproducible in different lots. A complex pattern of antioxidant/DNA repair gene expression emerged, reflecting the preponderance of seed lot-specific profiles. On...Continue Reading

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Datasets Mentioned

BETA
AB110609.1

Methods Mentioned

BETA
dissect
fluorescence spectroscopy
PCR

Software Mentioned

Primer3Plus
Rotor
REST2009
GeNorm
GraphPad Prism
Gene 6000 Series
GraphPad
Oligo Analyzer

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