Molecular envelope and atomic model of an anti-terminated glyQS T-box regulator in complex with tRNAGly

Nucleic Acids Research
Bhaskar Chetnani, Alfonso Mondragón

Abstract

A T-box regulator or riboswitch actively monitors the levels of charged/uncharged tRNA and participates in amino acid homeostasis by regulating genes involved in their utilization or biosynthesis. It has an aptamer domain for cognate tRNA recognition and an expression platform to sense the charge state and modulate gene expression. These two conserved domains are connected by a variable linker that harbors additional secondary structural elements, such as Stem III. The structural basis for specific tRNA binding is known, but the structural basis for charge sensing and the role of other elements remains elusive. To gain new structural insights on the T-box mechanism, a molecular envelope was calculated from small angle X-ray scattering data for the Bacillus subtilis glyQS T-box riboswitch in complex with an uncharged tRNAGly. A structural model of an anti-terminated glyQS T-box in complex with its cognate tRNAGly was derived based on the molecular envelope. It shows the location and relative orientation of various secondary structural elements. The model was validated by comparing the envelopes of the wild-type complex and two variants. The structural model suggests that in addition to a possible regulatory role, Stem III could ...Continue Reading

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Citations

Nov 20, 2019·Nature Structural & Molecular Biology·Shuang LiJinwei Zhang
May 16, 2018·Nature Communications·Krishna C SuddalaNils G Walter
Mar 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Anna V SherwoodTina M Henkin
Jul 28, 2018·Microbiology Spectrum·Kiel D Kreuzer, Tina M Henkin

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Methods Mentioned

BETA
in vitro transcription
nuclear magnetic resonance
X-ray
size-exclusion chromatography
gel filtration
light scattering
aminoacylation

Software Mentioned

SUPCOMB
AquaSAXS
RNAComposer
CNS
ASTRA
CRYSOL
DAMAVER
PHENIX
PyMOL
DAMMIN

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