Molecular epidemiological analysis and risk factors for acquisition of carbapenemase-producing Enterobacter cloacae complex in a Japanese university hospital

Antimicrobial Resistance and Infection Control
Nobuyuki TetsukaTetsuya Yagi

Abstract

To clarify the molecular epidemiology of carbapenem-resistant Enterobacter cloacae complex (CREC) and the risk factors for acquisition of carbapenemase-producing E. cloacae complex (CPEC). Using clinical CREC isolates detected in a Japanese university hospital over 4 years, carbapenemase production was screened with phenotypic methods. Carbapenemase genes were analysed by PCR and sequencing. Molecular epidemiological analyses were conducted with repetitive extragenic palindromic (REP)-PCR and multilocus sequence typing (MLST). CRECs were identified to the subspecies level by hsp60 sequencing. Whole-genome sequencing of plasmids was conducted. A case-control study was performed to identify risk factors for acquisition of CPEC among patients with CREC. Thirty-nine CRECs including 20 CPECs carrying blaIMP-1 were identified. Patients with CPEC had longer hospital stay before detection (26.5 days vs. 12 days, p = 0.008), a urinary catheter (odds ratio [OR], 5.36; 95% confidence interval [CI], 1.14-30.9; p = 0.023), or intubation (OR, 7.53; 95% CI, 1.47-53.8; p = 0.008) compared to patients without CPEC. Four genetically closely related CPEC clusters were observed, which showed that three of four CPEC clusters corresponded to E. asbu...Continue Reading

References

Sep 6, 2003·Applied and Environmental Microbiology·Harald Hoffmann, Andreas Roggenkamp
Jun 19, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Patricia A BradfordJohn Quale
Jun 10, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Neil GuptaAlexander J Kallen
Jun 19, 2013·PloS One·Tohru Miyoshi-AkiyamaTeruo Kirikae
Jun 17, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P Nordmann, L Poirel
Mar 5, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Hajime KanamoriWilliam A Rutala
Dec 26, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pranita D TammaPatricia J Simner
Jun 9, 2017·PLoS Computational Biology·Ryan R WickKathryn E Holt
Oct 19, 2017·Antimicrobial Agents and Chemotherapy·Karlijn van LoonMargreet C Vos
Jan 10, 2018·Antimicrobial Agents and Chemotherapy·Kotaro AokiKazuhiro Tateda
May 19, 2018·Emerging Infectious Diseases·Gisele PeiranoJohann D D Pitout
Jun 27, 2018·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Daizo KatoTetsuya Yagi
Aug 22, 2018·Current Infectious Disease Reports·Leighanne O Parkes, Susy S Hota

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Citations

Oct 22, 2019·Current Opinion in Gastroenterology·Jasper P VinkJonathan D Edgeworth
Jul 2, 2021·Japanese Journal of Infectious Diseases·Jayathilake SarangiYoshichika Arakawa

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Datasets Mentioned

BETA
PRJDB7521
SAMD00143514
SAMD00143520
AP019382
AP019388
AP081352.1

Methods Mentioned

BETA
PCR
electrophoresis
genotyping

Software Mentioned

ClustalW
Unicycler
Unicycler Genomics Workbench
Miniasm
BLAST
Sequencher
T346Hunter
DiversiLab
CPE Toolkits

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