Molecular Epidemiological Survey of Leptospira Infection of Wild Rodents in the Urban Settlement of Cambodia

Vector Borne and Zoonotic Diseases
Yoshiko KudoToshiyuki Masuzawa

Abstract

Leptospirosis remains a major public health threat in Cambodia. In this study, we aimed at facilitating the development of preventive strategies against leptospirosis in Cambodia by conducting molecular epidemiological surveys of Leptospira infection among wild rodents. One hundred sixty-three wild rodents were captured in the capital Phnom Penh and surrounding areas, and their kidneys and bladders were collected for analysis. Identification of wild rodent species was determined by using the cytochrome c oxidase I gene. TaqMan PCR of the flagellin B gene (flaB) was performed to detect Leptospira, and species of the isolates were identified by flaB sequencing analysis. The species and respective number of rodents collected were as follows: Rattus norvegicus, 80 (49.1%); Rattus argentiventer, 53 (32.5%); Rattus exulans, 6 (3.7%); Rattus indica, 15 (9.2%); Maxomys surifer, 3 (1.8%); and Rattus sp., 6 (3.7%). Leptospira was detected in 20 out of the 163 rodents (12.3%) and was categorized as either Leptospira interrogans or Leptospira noguchii. R. norvegicus had the highest prevalence of Leptospira (17.5%), and R. argentiventer and Rattus sp. showed infection rates of 9.4% and 16.7%, respectively. Based on these results, wild roden...Continue Reading

References

Nov 1, 2002·The American Journal of Tropical Medicine and Hygiene·Kanti LarasAndrew L Corwin
Apr 7, 2009·Veterinary Microbiology·Ben Adler, Alejandro de la Peña Moctezuma
Aug 7, 2010·International Journal of Antimicrobial Agents·Bernadette Abela-RidderRudy A Hartskeerl
Mar 19, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R A HartskeerlW A Ellis
Jun 6, 2012·The American Journal of Tropical Medicine and Hygiene·Svilena IvanovaSerge Morand
Jun 6, 2014·PLoS Neglected Tropical Diseases·Jean-François CossonSerge Morand
Jan 30, 2015·Vector Borne and Zoonotic Diseases·Hoang Kim LoanJuan J Carrique-Mas

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Citations

Feb 1, 2019·Transboundary and Emerging Diseases·Anahi S VieiraWalter Lilenbaum
Feb 28, 2019·PLoS Neglected Tropical Diseases·Kim R BlasdellCadhla Firth
Aug 10, 2019·PLoS Neglected Tropical Diseases·Kenneth BoeySreekumari Rajeev
Nov 7, 2021·Transboundary and Emerging Diseases·Luiza AyméeWalter Lilenbaum

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

MegAlign
SPSS
Basic Local Alignment Search Tool ( BLAST )
Clustal W
BLAST

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