Molecular epidemiology and in-vitro antifungal susceptibility of Aspergillus terreus species complex isolates in Delhi, India: evidence of genetic diversity by amplified fragment length polymorphism and microsatellite typing

PloS One
Shallu KathuriaAnuradha Chowdhary

Abstract

Aspergillus terreus is emerging as an etiologic agent of invasive aspergillosis in immunocompromised individuals in several medical centers in the world. Infections due to A. terreus are of concern due to its resistance to amphotericin B, in vivo and in vitro, resulting in poor response to antifungal therapy and high mortality. Herein we examined a large collection of molecularly characterized, geographically diverse A. terreus isolates (n = 140) from clinical and environmental sources in India for the occurrence of cryptic A. terreus species. The population structure of the Indian A. terreus isolates and their association with those outside India was determined using microsatellite based typing (STR) technique and Amplified Fragment Length Polymorphism analysis (AFLP). Additionally, in vitro antifungal susceptibility of A. terreus isolates was determined against 7 antifungals. Sequence analyses of the calmodulin locus identified the recently described cryptic species A. hortai, comprising 1.4% of Aspergillus section Terrei isolates cultured from cases of aspergilloma and probable invasive aspergillosis not reported previously. All the nine markers used for STR typing of A. terreus species complex proved to be highly polymorphi...Continue Reading

References

Jul 1, 1995·The Journal of Allergy and Clinical Immunology·R D deShazo, R E Swain
Apr 1, 1995·Applied and Environmental Microbiology·N L Glass, G C Donaldson
Dec 24, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C NeuvégliseJ P Latgé
Dec 4, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S AsciogluUNKNOWN Mycoses Study Group of the National Institute of Allergy and Infectious Diseases
Dec 10, 2003·Journal of Clinical Microbiology·John W BaddleyStephen A Moser
Aug 26, 2004·Antimicrobial Agents and Chemotherapy·William J SteinbachDaniel K Benjamin
Oct 4, 2005·British Journal of Haematology·Cornelia Lass-FlörlDavid Nachbaur
Nov 3, 2005·Evolution; International Journal of Organic Evolution·A PringleJ W Taylor
May 26, 2006·Mycologia·Seung-Beom HongRobert A Samson
Jan 24, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Arlo UptonKieren A Marr
Mar 23, 2007·Journal of Clinical Microbiology·J M BainF C Odds
Jun 22, 2007·Journal of Clinical Microbiology·Cornelia Lass-FlörlDimitrios P Kontoyiannis
Dec 17, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·G BlumC Lass-Flörl
Mar 24, 2009·Eukaryotic Cell·S Arunmozhi BalajeeUNKNOWN ISHAM Working Group on A. terreus
Mar 29, 2011·The Journal of Hospital Infection·M J G T RüpingO A Cornely
Sep 6, 2011·Studies in Mycology·R A SamsonJ Varga
Jul 12, 2012·The Journal of Infectious Diseases·Maiken C ArendrupCornelia Lass-Flörl
Oct 18, 2012·Mycologia·Michael Arabatzis, Aristea Velegraki
Feb 7, 2013·Critical Reviews in Microbiology·Anuradha ChowdharyJacques F Meis
Jun 14, 2013·Medical Mycology·Wolfgang MutschlechnerCornelia Lass-Flörl
Aug 10, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A RougeronC H W Klaassen

❮ Previous
Next ❯

Citations

Jan 27, 2016·Mycoses·Sarah Santos GonçalvesArnaldo Lopes Colombo
Nov 10, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Anupam PrakashAnuradha Chowdhary
Nov 19, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Michaela LacknerCornelia Lass-Flörl
May 11, 2016·Expert Review of Clinical Pharmacology·Laura L KovandaWilliam W Hope
Aug 5, 2015·Antimicrobial Agents and Chemotherapy·Anuradha ChowdharyJacques F Meis
May 12, 2017·Clinical Microbiology Reviews·Alexandre AlanioStéphane Bretagne
Apr 29, 2016·PLoS Pathogens·Anuradha ChowdharyJacques F Meis
Jun 14, 2019·Journal of Clinical Microbiology·Huilin SuSybren de Hoog
Mar 19, 2020·Emerging Infectious Diseases·Anuradha ChowdharyJacques F Meis
Aug 27, 2019·Mycology·Sonia Kumari ShishodiaJata Shankar
Jul 22, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Eemke L AssendorpStefanie S V Henriet
Apr 13, 2018·Frontiers in Microbiology·Tamara ZoranMichaela Lackner
Jul 4, 2016·Journal of Fungi·Olga Rivero-MenendezManuel Cuenca-Estrella
Dec 6, 2018·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Tobias G P EngelUNKNOWN Dutch Cystic Fibrosis Fungal Collection Consortium
Jun 3, 2020·Journal of Proteomics·Sonia K Shishodia, Jata Shankar
Apr 4, 2021·Microorganisms·Anna-Maria DietlCornelia Lass-Flörl
Jul 1, 2021·Clinical Microbiology Reviews·Cornelia Lass-FlörlMatthias Brock

❮ Previous
Next ❯

Datasets Mentioned

BETA
EU147582

Methods Mentioned

BETA
PCR
genotyping
restriction ligation
Enzyme-linked Immunosorbent assay
ELISA
lavage

Software Mentioned

UPGMA
ClustalW
Sequencing Analysis
MEGA
BLAST

Related Concepts

Related Feeds

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.