Molecular epidemiology of carbapenemase-producing Escherichia coli and the prevalence of ST131 subclone H30 in Shanghai, China

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
F ZhangJ Sun

Abstract

The molecular characteristics and epidemiology of carbapenemase-producing Escherichia coli (CPEC) isolates from Shanghai, China, were investigated using 21 imipenem-resistant E. coli isolates obtained from a Shanghai teaching hospital from 2011 to 2014. The presence of bla KPC, bla IMP, bla VIM, bla OXA-48, and bla NDM was assessed by polymerase chain reaction (PCR) amplification and sequencing. CPEC isolates were characterized by the Etest®, multilocus sequence typing (MLST), and pulse-field gel electrophoresis (PFGE). Plasmids carrying resistance genes were analyzed by conjugation experiments, replicon typing, plasmid MLST (pMLST), S1 nuclease PFGE (S1-PFGE), and Southern hybridization. The genetic environment of the resistance genes was determined by PCR and sequencing. Among the 21 E. coli isolates, 16 produced carbapenemases; of these, ten isolates transferred carbapenemase-encoding plasmids to recipient bacteria. Nine of the 16 isolates were clonally related, and their PFGE patterns were designated type A. ST131 was the predominant sequence type (11 isolates, 68.8 %); the H30 subclone comprised 81.8 % of the ST131 strains. In all three isolates, bla IMP-4 was located on 50-kb IncN plasmids. All but two bla KPC-2 genes wer...Continue Reading

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Citations

May 25, 2016·International Journal of Antimicrobial Agents·Lian-Hui WangYu-Juan Zhang
May 6, 2016·The Journal of Antimicrobial Chemotherapy·Adriana OrtegaUNKNOWN Spanish Collaborating Group for the Antibiotic Resistance Surveillance Programme
Sep 7, 2016·Virulence·David van Duin, Yohei Doi
Jun 2, 2016·Antimicrobial Agents and Chemotherapy·Nicole StoesserJohann D Pitout
Aug 11, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Bente Olesen
Nov 30, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Marie-Hélène Nicolas-ChanoineUNKNOWN “E-carb Study Group”

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