Molecular epidemiology of Cryptosporidium infection in infants with diarrhea in Wuhan City

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control
Liu Xiao-JieZhou Rui

Abstract

To understand the prevalence of Cryptosporidium infection in diarrhea infants under 2 years old in Wuhan City, so as to provide the epidemiological evidence for the prevention and treatment of cryptosporidiosis. The fecal samples from infants under 2 years old with diarrhea were collected in Hubei General Hospital and Central South Hospital in Wuhan City, Hubei Province from August 2014 to July 2015. The fecal samples were stored in 2.5% potassium dichromate at 4 ℃ after filtered. The DNA was extracted from the fecal pellets with the phenol-chloroform method. The Cryptosporidium species were detected by a nested PCR assay targeting the SSU rRNA gene of the parasite. All the positive PCR products were sequenced on ABI 3100 automated sequencer, and the amplified sequences were compared to homologous sequences in the NCBI database by using the Basic Local Alignment Search Tool (BLAST). Phylogenetic analyses were performed by using the software MEGA (version 4.0) based on the Neighbour-Joining method. The human stool specimens (n = 298) were screened for the presence of Cryptosporidium by nested PCR. The infection rate of Cryptosporidium was 3.02% (9/298). The infection rate of Cryptosporidium was 5.93% (7/118) in the infants betwe...Continue Reading

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