Molecular epidemiology of human picobirnaviruses among children of a slum community in Kolkata, India

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Rittwika BhattacharyaTriveni Krishnan

Abstract

Picobirnaviruses are a group of unclassified, non-enveloped, small spherical viruses, 35-41 nm in diameter without any apparent surface morphology. They have characteristic bisegmented double stranded RNA genome of two types namely large profile (2.3-2.6 kbp for the larger and 1.5-1.9 kbp for the smaller segment, respectively) or small profile (1.75 and 1.55 kbp for segments 1 and 2, respectively). Human picobirnaviruses (n=12 positives; 2/56 diarrhoeic children and 10/607 non-diarrhoeic children) with large (n=11) or small (n=1) genome pattern were observed in faecal specimens of children from a slum community by silver stained PAGE gels. Faecal specimen from four asymptomatic cases (P597_02_IND, K135_02_IND, A373_03_IND, A356_03_IND) and one diarrhoeic case (K135_03_IND) had genogroup I picobirnaviruses (1-CHN-97 like) showing amplicons within the 201 bp region, with primers PicoB25-PicoB43, targeting the conserved domain of RNA-dependent RNA polymerase (RdRp) gene. It was interesting to note that only the PBV strain P597_02_IND from Kolkata with large genome was closely related to a reported strain (similarity with 2-GA-91 from USA was 87% at the nucleotide level and 90% at the amino acid level). Sequence analysis showed thr...Continue Reading

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Citations

Nov 3, 2009·Archives of Virology·Maria Clara Duarte Fregolente, Maria Silvia Viccari Gatti
Jan 7, 2009·Applied and Environmental Microbiology·Erin M SymondsMya Breitbart
May 30, 2008·Journal of Clinical Microbiology·Gabriela M CarruyoJuan E Ludert
Mar 19, 2011·Veterinary Research·Balasubramanian GaneshNobumichi Kobayashi
Sep 13, 2011·PLoS Pathogens·Tung G PhanEric L Delwart
Feb 13, 2015·Virusdisease·Balasubramanian GaneshZornitsa Mladenova
Jul 6, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Laura C MartínezSilvia V Nates
May 26, 2009·Virus Research·Maria Clara Duarte FregolenteMaria Silvia Viccari Gatti
Feb 9, 2012·Reviews in Medical Virology·Balasubramanian GaneshNobumichi Kobayashi
Jan 18, 2015·Journal of Medical Microbiology·Zornitsa MladenovaMiren Iturriza-Gomara
Oct 11, 2013·The Journal of General Virology·René Ribeiro SilvaJoana D'Arc Pereira Mascarenhas
Feb 25, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Balasubramanian GaneshTriveni Krishnan
Dec 2, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Gisela MasachessiSilvia V Nates
Oct 6, 2018·The Open Virology Journal·Yashpal Singh MalikRaj Kumar Singh
Dec 30, 2016·Archives of Virology·Lauren WilburnNiwat Maneekarn

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