Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus in the Ovine Dairy Chain and in Farm-Related Humans

Toxins
Guerrino MacoriL Decastelli

Abstract

Staphylococcus aureus is a major cause of clinical infections in humans and its enterotoxins cause foodborne disease. In the present study, we tested a total of 51 isolates of S. aureus from small-ruminant dairy farms with artisan dairy facilities, all located in Latium, Italy. The farms have a known history of a high prevalence of methicillin-resistant S. aureus (MRSA). Most of the MRSA isolates (27 of 51) belonged to spa-type t127 (43.1%), followed by t2678 (3.9%), t044 (2%), t1166 (2%), and t1773 (2%). PFGE performed on mecA positive strains identified one cluster (≥ 80% of similarity), comprising 22 MRSA. Nine of twenty-two MRSA isolates were assigned human host origin, and 13 isolates did not belong to a specific host. During the characterization study, one strain isolated from bulk tank milk samples harbored the pvl gene; the strain was not enterotoxigenic with a non-specific host according to the biotyping scheme, highlighting the possible emerging risk of transmission of bacterial virulence factors by foods, the environment, and foodhandlers. These findings stress the importance of hygienic measures at all processing steps of the food production chain and underline that monitoring for the presence of MRSA throughout the...Continue Reading

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Citations

Oct 14, 2018·International Journal of Environmental Research and Public Health·Abongile Pekana, Ezekiel Green
Feb 27, 2019·EFSA Journal·UNKNOWN European Food Safety Authority, UNKNOWN European Centre for Disease Prevention and Control
Sep 3, 2020·EFSA Journal·UNKNOWN European Food Safety Authority, UNKNOWN European Centre for Disease Prevention and Control
Jan 8, 2020·BMC Veterinary Research·Katerina NeradovaHelena Zemlickova
Sep 22, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mary LuceyKirsten Schaffer
Apr 20, 2021·EFSA Journal·UNKNOWN European Food Safety Authority, UNKNOWN European Centre for Disease Prevention and Control

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Methods Mentioned

BETA
electrophoresis
biotyping
PCR

Software Mentioned

BioNumerics

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