Abstract
Tuberculosis has had significant effects on Ireland over the past two centuries, causing persistently higher morbidity and mortality than in neighbouring countries until the last decade. This study describes the results of genotyping and drug susceptibility testing of 171 strains of Mycobacterium tuberculosis complex isolated between January 2004 and December 2006 in a region of Ireland centred on the city of Cork. Spoligotype comparisons were made with the SpolDB4 database and clustered 130 strains in 23 groups, forty-one strains showed unique Spoligotyping patterns. The commonest spoligotypes detected were ST0137 (X2) (16.9%), and ST0351 (15.8%) ('U' clade). The major spoligotype clades were X (26.2%), U (19.3%), T (15.2%), Beijing (5.9%), Haarlem (4.7%), LAM (4.1%), BOVIS (1.75%), with 12.9% unassigned strains. A 24-locus VNTR genotyping produced 15 clusters containing 49 isolates, with high discrimination index (HGDI>0.99). A combination of Spoligotyping and VNTR reduced the number of clustered isolates to 47 in 15 clusters (27.5%). This study identified ST351 as common among Irish nationals, and found a low rate of drug resistance with little evidence of transmission of drug resistant strains. Strain clustering was signifi...Continue Reading
References
Mar 1, 1992·Irish Journal of Medical Science·M G Cormican, J Flynn
Jun 16, 1994·The New England Journal of Medicine·P M SmallG K Schoolnik
Jun 5, 1998·Microbiology·Richard Frothingham, Winifred A Meeker-O'Connell
Apr 14, 1999·The British Journal of Surgery·M LamahJ A Dormandy
Apr 3, 2001·Journal of Clinical Microbiology·C SolaN Rastogi
Feb 5, 2002·Journal of Clinical Microbiology·Malcolm D YatesStuart M Wilson
Jul 26, 2002·The Journal of Biological Chemistry·Patricia ConstantChristophe Guilhot
Sep 14, 2002·Statistics in Medicine·David W Hosmer, Nils Lid Hjort
Nov 28, 2002·Emerging Infectious Diseases·Ingrid FilliolNalin Rastogi
Apr 3, 2003·Journal of Bacteriology·Jeremy W DaleHasan Yesilkaya
Dec 8, 2005·Applied and Environmental Microbiology·Andrea GibsonFrancis Drobniewski
Jul 11, 2006·Journal of Clinical Microbiology·Krishna K GopaulFrancis A Drobniewski
Sep 29, 2006·Journal of Clinical Microbiology·Philip SupplyDick van Soolingen
Dec 29, 2006·Journal of Clinical Microbiology·Mara Cardoso OelemannPhilip Supply
Aug 4, 2007·American Journal of Respiratory and Critical Care Medicine·Ulf R DahleEinar Heldal
Feb 1, 2008·Journal of Clinical Microbiology·Caroline Allix-BéguecPhilip Supply
Dec 3, 2008·Emerging Infectious Diseases·Olabisi OjoMichael B Prentice
Feb 5, 2009·Archives of Disease in Childhood·J T GaensbauerM Ní Chróinín
Oct 14, 2009·PloS One·Stefan NiemannJohn A C Archer
Citations
May 25, 2011·American Journal of Epidemiology·Martien W BorgdorffDick van Soolingen
Jan 23, 2015·BMJ Open·Jessica MearsPam Sonnenberg
Mar 4, 2015·PloS One·Takayuki WadaTaro Yamamoto
Feb 6, 2017·ERJ Open Research·Catherine M SmithAndrew C Hayward
May 21, 2017·Biomédica : revista del Instituto Nacional de Salud·Claudia CastroGloria Puerto
Apr 5, 2019·Journal of Clinical Microbiology·R C JonesA P Davies