PMID: 7541747Mar 1, 1995Paper

Molecular epidemiology of respiratory syncytial virus: a review of the use of reverse transcription-polymerase chain reaction in the analysis of genetic variability

Electrophoresis
P A Cane, C R Pringle

Abstract

Respiratory syncytial virus (RSV) is the major viral cause of lower respiratory tract disease (bronchiolitis and pneumonia) in babies and infants. Infections with the virus occur as annual winter epidemics in temperate climates, placing considerable pressure on the provision of hospital beds. The virus is unusual in that it can reinfect individuals and it can infect babies despite the presence of maternal antibody. RSV has a negative sense nonsegmented RNA genome and as such is liable to high levels of mutation. This paper describes methods developed to determine the degree of genetic variability of the virus both during individual epidemics and worldwide. It is necessary for these methods to be quick, easy and cheap so that large numbers of samples can be analysed readily. They are based on extraction of viral RNA directly from clinical samples or from viral cultures, reverse transcription of the viral RNA, and then amplification of selected regions of the genome by the polymerase chain reaction (PCR). PCR products are then analysed by restriction mapping, or, if necessary, direct nucleotide sequencing. In this way isolates of RSV have been shown to fall into a number of genotypes, with epidemics being made up of cocirculating...Continue Reading

References

Dec 1, 1992·Journal of Virological Methods·P A Cane, C R Pringle
Apr 1, 1991·The Journal of Infectious Diseases·L J AndersonK McIntosh
Apr 1, 1991·The Journal of Infectious Diseases·G A StorchD E Dohner
Oct 1, 1985·The Journal of General Virology·M A MufsonE Norrby
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P R JohnsonP L Collins
Apr 1, 1985·The Journal of Infectious Diseases·L J AndersonK McIntosh
May 1, 1986·The Journal of General Virology·H B GimenezP Cash
Jan 1, 1994·Journal of Clinical Microbiology·P A CaneC R Pringle

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Citations

Apr 12, 2002·Pediatric Pulmonology·Rosalind L SmythC Anthony Hart
Jun 18, 2004·Emerging Infectious Diseases·Bernadette G van den HoogenRon A M Fouchier
Sep 7, 2006·Journal of Clinical Microbiology·Shama ParveenShobha Broor
Jan 29, 2008·Current Biology : CB·Sophie KöndgenFabian H Leendertz
Apr 29, 1998·Journal of Medical Virology·A RohwedderH Werchau
Aug 8, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N A TerraultT L Wright

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