Molecular Epidemiology of Shiga Toxin-Producing Escherichia coli (STEC) on New Zealand Dairy Farms: Application of a Culture-Independent Assay and Whole-Genome Sequencing

Applied and Environmental Microbiology
A Springer BrowneN P French

Abstract

New Zealand has a relatively high incidence of human cases of Shiga toxin-producing Escherichia coli (STEC), with 8.9 STEC cases per 100,000 people reported in 2016. Previous research showed living near cattle and contact with cattle feces as significant risk factors for STEC infections in humans in New Zealand, but infection was not linked to food-associated factors. During the 2014 spring calving season, a random, stratified, cross-sectional study of dairy farms (n = 102) in six regions across New Zealand assessed the prevalence of the "Top 7" STEC bacteria (serogroups O157, O26, O45, O103, O111, O121, and O145) in young calves (n = 1,508), using a culture-independent diagnostic test (PCR/MALDI-TOF). Twenty percent (306/1,508) of calves on 75% (76/102) of dairy farms were positive for at least one of the "Top 7" STEC bacteria. STEC carriage by calves was associated with environmental factors, increased calf age, region, and increased number of calves in a shared calf pen. The intraclass correlation coefficient (ρ) indicated strong clustering of "Top 7" STEC-positive calves for O157, O26, and O45 serogroups within the same pens and farms, indicating that if one calf was positive, others in the same environment were likely to b...Continue Reading

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Citations

Nov 16, 2019·Journal of Translational Medicine·Zelin CuiLi Li
Mar 28, 2021·Applied and Environmental Microbiology·A Springer BrowneNigel P French
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Nicolás GalarceNilton Lincopan

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Nullarbor
Inkscape
R
CLUSTER
randomForest
Interactive Tree of Life ( iTOL )
aod package
PRIMER
PERMANOVA
RaxML

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