Molecular epidemiology of Staphylococcus aureus in food samples based on the protein A gene polymorphic region DNA sequence

Canadian Journal of Microbiology
M M Soltan-DallalJalil F Mehrabadi

Abstract

Foodborne disease due to Staphylococcus aureus is a common and important disease worldwide. Molecular typing of S. aureus strains plays a crucial role in epidemiological studies examining the origin and performing surveillance of major infections. In this survey, we collected 913 food samples and detected 93 S. aureus isolates by using culture and biochemical tests. Subsequently, the X region of the protein A gene was amplified by the polymerase chain reaction (PCR) and amplicons were digested with HaeII and HindIII. Seven different patterns, ranging in length between 1200 and 1600 bp, showed gene polymorphism of the spa gene. The most prevalent spa types were D (20%) and C (16%) in dairy products and D (6%) and E (3%) in meat products. Consequently, 16 genotypes were obtained by HaeII digestion. Typeability of PCR - restriction fragment length polymorphism in genotyping of S. aureus strains in our study was perfect. Therefore, this method is a reliable, rapid, and powerful system in epidemiological investigations.

References

Jul 1, 1992·Journal of Clinical Microbiology·S H GohA W Chow
Jun 29, 2001·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·T A WichelhausV Brade
Oct 11, 2003·Veterinary Research Communications·R CascianoR Rosmini
Oct 26, 2006·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·C VimercatiP Moroni
Nov 6, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A van BelkumUNKNOWN European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group on Epidemiological Markers (ESGEM)
May 6, 2008·International Journal of Food Microbiology·Omer AkinedenEwald Usleber

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Citations

Dec 9, 2014·Jundishapur Journal of Microbiology·Hajar MadahiFarhad Safarpoor Dehkordi
Nov 6, 2014·Jundishapur Journal of Microbiology·Fatemeh ShakeriMaya Babai Kochkaksaraei

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