Molecular events of cellular apoptosis and proliferation in the early tendon healing period

The Journal of Hand Surgery
Ya Fang WuJin Bo Tang

Abstract

Cellular proliferation is accompanied by cellular apoptosis. In the healing digital flexor tendon, molecular events concerning cellular apoptosis have not been investigated. This study aimed to investigate the relationship between cellular apoptosis and proliferation in early tendon healing. The flexor digitorum profundus tendons of 50 long toes in 25 chickens were transected and were repaired surgically. On postoperative days 3, 7, 14, 21, and 28, we subjected tendons to in situ terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) assay to detect apoptotic cells, immunofluorescence staining with antibodies to proliferating cell nuclear antigen to assess proliferation, and Bcl-2, an anti-apoptotic protein, to assess responses suppressive to apoptosis. The positively labeled tenocytes were counted microscopically and compared statistically. We also stained sections with hematoxylin and eosin to observe their healing status. An additional 12 tendons (6 chickens) served as day 0 controls. Compared with tendons at day 0, the healing tendons had notably greater cellularity in both epitenon and endotenon areas. The total number of cells and number of TUNEL-positive cells peaked at day 3. At days 7 to 21, the number of ...Continue Reading

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Oct 12, 2012·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Kirsten LundgreenLars Engebretsen
Oct 5, 2010·The Journal of Hand Surgery·Maria E BerglundMonica E Wiig
Apr 13, 2011·British Journal of Sports Medicine·Kirsten LundgreenAlex Scott
Oct 30, 2012·Hand Clinics·Ren Guo Xie, Jin Bo Tang
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May 20, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Nelly Andarawis-PuriEvan L Flatow
May 21, 2020·Bioscience Reports·Wei Feng MaoYa Fang Wu
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May 8, 2021·Orthopaedic Journal of Sports Medicine·Tung-Yang YuWen-Chung Tsai

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