Molecular evidence for the involvement of alpha crystallin in the colouration/crosslinking of crystallins in age-related nuclear cataract

Experimental Eye Research
Y C ChenR J Truscott

Abstract

The proteins of the lens which become insoluble, crosslinked and coloured as a result of the onset of human nuclear cataract have been studied using a combination of enzymatic digestion and HPLC/mass spectrometry (MS). The objective was to determine if such an approach could provide information on the identities of the polypeptide components involved in the colouration and crosslinking and to discover whether any crystallins predominate in this characteristic post-translational modification process. Initially, coloured high molecular weight peptides were isolated from a tryptic/chymotryptic digest of the 6 M guanidine hydrochloride-insoluble lens protein fraction. These tryptic/chymotryptic peptides were then incubated with pronase and the small peptides released, purified by gel filtration. All but one of the peptides analysed by HPLC/MS/MS were found to contain proline. Peptides derived from alpha-crystallin were found to comprise the great majority of the peptides characterised. No gamma-crystallin peptides were observed. Both alpha A-crystallin and alpha B-crystallin were represented. Further, all but one of these peptides were derived from the N-terminal region of the alpha-crystallin subunits: a region recently implicated...Continue Reading

References

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Citations

Jun 26, 2001·Photochemistry and Photobiology·L A ErvinE R Gaillard
Oct 8, 2009·Brain Research Bulletin·Alexei Surguchev, Andrei Surguchov
Feb 12, 2011·Biotechnology Progress·Rachael A LewusStanley I Sandler
Aug 11, 2005·Analytical Biochemistry·Wai-Kei ShumKevin M Downard
Mar 14, 2000·Experimental Eye Research·F A ShamsiR H Nagaraj
May 17, 2011·Cell Biochemistry and Biophysics·Aabgeena Naeem, Naveed Ahmad Fazili
Nov 8, 2014·Biotechnology Progress·Rachael A LewusStanley I Sandler
Feb 12, 2021·Chembiochem : a European Journal of Chemical Biology·Megan A RochaRachel W Martin
Mar 8, 2013·Experimental Eye Research·Rebecca S McGrealMarc Kantorow
Apr 17, 2003·Experimental Eye Research·Paulo PereiraAllen Taylor
May 3, 2005·Experimental Eye Research·Roger J W Truscott

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