Molecular Evidence of Different Rickettsia Species in Villeta, Colombia

Vector Borne and Zoonotic Diseases
Álvaro A Faccini-MartínezMarylin Hidalgo

Abstract

The aim of this work was to detect and identify Rickettsia species in ticks collected in rural areas of Villeta, Colombia. Tick specimens were collected from domestic animals and walls of houses in five rural villages of Villeta town and from humans in Naranjal village (same town). Moreover, a flea collected from the same area was also processed. DNA was extracted and tested by conventional, semi-nested, and nested PCR reactions targeting rickettsial genes. In the ticks collected from humans from Naranjal village, a nymph of Amblyomma cajennense sensu lato was amplified using primers for ompA and sequenced (100% identity with "Candidatus Rickettsia amblyommii"). Last, three amplicons from the Ctenocephalides felis flea, corresponding to gltA, ompB, and 16S rRNA genes, showed high identity with R. felis (98.5%, 97.3%, and 99.2%, respectively) and "Candidatus Rickettsia asemboensis" (99.7% and 100%, respectively). To our knowledge, these results correspond to the first molecular detection in Colombia of "Candidatus Rickettsia amblyommii" and "Ca. Rickettsia asemboensis" in fleas.

References

Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
Mar 1, 1990·Journal of Clinical Microbiology·L WebbA F Azad
May 23, 1998·The American Journal of Tropical Medicine and Hygiene·F J MárquezM V Borobio
Dec 21, 2004·Journal of Medical Entomology·Marcelo B LabrunaDavid H Walker
Mar 9, 2005·Emerging Infectious Diseases·Yeon-Joo ChoiIk-Sang Kim
Feb 17, 2006·Annals of the New York Academy of Sciences·A PortilloJ A Oteo
Jan 25, 2008·Emerging Infectious Diseases·Marylin HidalgoGustavo Valbuena
Aug 4, 2009·Journal of Medical Entomology·Sergio E BermúdezGregory A Dasch
Sep 26, 2013·Future Microbiology·Sanjeev K SahniDavid H Walker

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Citations

Sep 13, 2016·Ticks and Tick-borne Diseases·Sokani Sánchez-MontesIngeborg Becker
Aug 11, 2016·International Journal of Systematic and Evolutionary Microbiology·Alice N MainaAllen L Richards
Dec 20, 2016·Vector Borne and Zoonotic Diseases·Yerlan SansyzbayevAllen L Richards
Sep 18, 2016·International Journal of Systematic and Evolutionary Microbiology·Sandor E KarpathyChristopher D Paddock
Apr 12, 2017·Journal of Medical Entomology·Ana M PalomarJavier Millán
Jul 20, 2017·The American Journal of Tropical Medicine and Hygiene·Álvaro A Faccini-MartínezMarylin Hidalgo
Sep 19, 2017·PLoS Neglected Tropical Diseases·Juan C Quintero VCarlos Rojas A
Oct 18, 2018·Cadernos de saúde pública·Marcela Patricia Eraso-CadenaLina Andrea Gutierrez-Builes
Mar 29, 2019·Parasites & Vectors·Ricardo G Maggi, Friederike Krämer
Jan 29, 2019·Frontiers in Veterinary Science·Alice N MainaAllen L Richards
Oct 17, 2019·Journal of Medical Entomology·Paola Betancourt-RuizMarylin Hidalgo
Feb 13, 2021·Research and Reports in Tropical Medicine·Maria A Caravedo MartinezLucas S Blanton
Nov 22, 2020·Emerging Infectious Diseases·Margarita ArboledaJuan D Rodas

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Methods Mentioned

BETA
PCR

Software Mentioned

BLAST

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