Molecular evidence of Helicobacter cinaedi organisms in human gastric biopsy specimens.

Journal of Clinical Microbiology
J A PeñaJ Versalovic

Abstract

One hundred twenty-six urease-negative gastric biopsy specimens were evaluated for the presence of Helicobacter genus-specific 16S ribosomal DNA (rDNA) and H. pylori-specific glmM DNA sequences by PCR. The species specificity of the glmM PCR assay was demonstrated, as H. pylori was the only Helicobacter species that yielded the expected glmM amplicon. Most urease-negative specimens (118 of 126 specimens) lacked Helicobacter DNA. However, 8 of 126 urease-negative specimens contained Helicobacter 16S rDNA. In order to identify the Helicobacter species present in urease-negative gastric biopsy specimens, 16S rDNA amplicons were cloned and sequenced. Sequence comparisons were performed by analyses of the sequences in public sequence databases. Two samples contained 16S rDNA that was identified as H. cinaedi with 100% identity and that spanned approximately 400 bp (398 and 398 bp, respectively). In contrast, multiple differences (97% identity; 390 of 398 bp) were observed with H. pylori 16S rDNA in this region. This finding was verified by sequencing an overlapping 537-bp fragment within the 5' portion of 16S rDNA. Although the clinical findings were consistent with H. pylori infection (e.g., duodenal ulcer disease), rapid urease te...Continue Reading

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Citations

Aug 29, 2002·Helicobacter·Stephen L W OnTorkel Wadström
Apr 13, 2007·Clinical Microbiology Reviews·Francis Mégraud, Philippe Lehours
May 25, 2006·BMC Infectious Diseases·Ilker UçkayVéronique Jacomo
Dec 24, 2003·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Hinako MurakamiTakashi Takahashi
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth M BikDavid A Relman
Mar 24, 2009·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·A Duquenoy, B Le Luyer
Dec 17, 2015·Internal Medicine·Satoshi MurataMasaki Tada
Jun 2, 2015·Internal Medicine·Yusaku AkashiMasatsune Suzuki
Sep 3, 2003·Alimentary Pharmacology & Therapeutics·S J BellM A Kamm
Nov 11, 2014·Helicobacter·Zeli ShenJames G Fox
Jun 25, 2013·FEMS Microbiology Reviews·Ines YangSebastian Suerbaum
Sep 2, 2016·Therapeutic Advances in Gastroenterology·Geoffrey A PreidisRobert J Shulman
Mar 28, 2002·Applied and Environmental Microbiology·Claudia M G HarperJames G Fox
Nov 18, 2003·Helicobacter·Hilpi RautelinFrancis Mégraud

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