DOI: 10.1101/503219Dec 21, 2018Paper

Molecular Evolution of Pseudomonas syringae Type III Secreted Effector Proteins

BioRxiv : the Preprint Server for Biology
Marcus M DillonDavid S Guttman

Abstract

Diverse Gram-negative pathogens like Pseudomonas syringae employ type III secreted effector (T3SE) proteins as primary virulence factors that combat host immunity and promote disease. T3SEs can also be recognized by plant hosts and activate an effector triggered immune (ETI) response that shifts the interaction back towards plant immunity. Consequently, T3SEs are pivotal in determining the virulence potential of individual P. syringae strains, and ultimately restrict P. syringae pathogens to a subset of potential hosts that are unable to recognize their repertoires of T3SEs. While a number of effector families are known to be present in the P. syringae species complex, one of the most persistent challenges has been documenting the complex variation in T3SE contents across a diverse collection of strains. Using the entire pan-genome of 494 P. syringae strains isolated from more than 100 hosts, we conducted a global analysis of all known and putative T3SEs. We identified a total of 14,613 T3SEs, 4,636 of which were unique at the amino acid level, and show that T3SE repertoires of different P. syringae strains vary dramatically, even among strains isolated from the same hosts. We also find that dramatic diversification has occurre...Continue Reading

Related Concepts

Alleles
Genes
Genome
Gram-Negative Bacteria
Virulence
Evolution, Molecular
Pseudomonas syringae
Syringa
Gene Transfer, Horizontal
Analysis

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