Abstract
Rifampicin resistance has arisen in several different species of bacteria because of alterations to one or more regions in the target of the antibiotic, the beta-subunit of RNA polymerase encoded by rpoB. Nucleotide sequence analysis of a 270 bp fragment of rpoB from 16 clinical rifampicin-susceptible isolates of Streptococcus pneumoniae, 8 clinical rifampicin-resistant isolates, and 3 spontaneous rifampicin-resistant mutants, has revealed that, as with previously examined species, point mutations within the cluster I region of rpoB, at sites encoding Asp516 and HiS526, also confer resistance to rifampicin in this important human pathogen. Moreover, the residues within cluster I, that were altered within the rifampicin-resistant mutants of S. pneumoniae, were in the same position as those previously found to alter in resistant isolates of Escherichia coli and Mycobacterium tuberculosis. Sequence analysis of rpoB, both from these isolates of S. pneumoniae and from two strains of S. mitis, reveals that, among a number of clinical isolates, resistance to rifampicin in S. pneumoniae has arisen by point mutation. However, the nucleotide sequence of rpoB from one isolate examined suggests that interspecies gene transfer may also have...Continue Reading
References
Oct 5, 1978·The New England Journal of Medicine·M R JacobsR Austrian
Nov 12, 1977·Lancet·P C AppelbaumR C Cooper
Aug 1, 1992·Molecular Microbiology·J Zhou, B G Spratt
Jul 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J LiñaresR Martin
Sep 1, 1991·Molecular Microbiology·T J CoffeyJ M Musser
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C G DowsonB G Spratt
Nov 20, 1988·Journal of Molecular Biology·D J JinC A Gross
Jul 5, 1988·Journal of Molecular Biology·D J Jin, C A Gross
Mar 10, 1988·Nature·B G Spratt
Sep 18, 1969·The New England Journal of Medicine·W B Deal, E Sanders
Jun 1, 1995·Antimicrobial Agents and Chemotherapy·T J CoffeyB G Spratt
Apr 1, 1994·Journal of Clinical Microbiology·V KapurJ M Musser
Apr 1, 1994·Journal of Clinical Microbiology·J F SessegoloL M Teixeira
Sep 1, 1994·Journal of Bacteriology·R HakenbeckT Grebe
Jun 1, 1994·Antimicrobial Agents and Chemotherapy·P E CarterT H Pennington
Apr 15, 1994·Science·B G Spratt
Feb 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C C John
Oct 1, 1993·Antimicrobial Agents and Chemotherapy·A TelentiT Bodmer
Jul 1, 1993·FEMS Microbiology Letters·T J CoffeyB G Spratt
Aug 1, 1993·Molecular Microbiology·C G DowsonR A Whiley
Mar 1, 1993·Antimicrobial Agents and Chemotherapy·N Honore, S T Cole
Sep 1, 1995·Antimicrobial Agents and Chemotherapy·K P KlugmanJ S Bradley
May 1, 1996·Antimicrobial Agents and Chemotherapy·G V DoernA M Rauch
Mar 1, 1996·Journal of Clinical Microbiology·A PascualE J Perea
Dec 1, 1996·Antimicrobial Agents and Chemotherapy·K P KlugmanA Bryskier
May 1, 1997·Chemotherapy·C FlorezE Martin-Mazuelos
Oct 23, 1997·Antimicrobial Agents and Chemotherapy·J P MaskellL M Hall
Citations
Feb 17, 2001·FEMS Microbiology Letters·J de VriesW Wackernagel
Sep 2, 1999·Infectious Disease Clinics of North America·K P Klugman, S A Madhi
Sep 11, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P M van TilburgP W Hermans
Nov 3, 2010·Antimicrobial Agents and Chemotherapy·María-José FerrándizAdela G de la Campa
Sep 26, 2012·Antimicrobial Agents and Chemotherapy·Aashish SrivastavaRichard H Ebright
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·N DohertyC G Dowson
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·A O'NeillI Chopra
Nov 18, 2000·Antimicrobial Agents and Chemotherapy·M MaurinD Raoult
Apr 18, 2002·Antimicrobial Agents and Chemotherapy·Hélène Aubry-DamonPatrice Courvalin
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Max MaurinJ Stephen Dumler
Feb 27, 2003·Antimicrobial Agents and Chemotherapy·Patricia Stutzmann MeierKathrin Mühlemann
Mar 26, 2003·Antimicrobial Agents and Chemotherapy·Antonio J Martín-Galiano, Adela G de la Campa
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·Wafa AchourAssia Ben Hassen
May 27, 2005·Antimicrobial Agents and Chemotherapy·María José FerrándizUNKNOWN Spanish Pneumococcal Infection Study Network
Jun 15, 2000·Journal of Bacteriology·M M Samrakandi, F Pasta
Jan 29, 2000·Journal of Bacteriology·J MajewskiC G Dowson
Jun 28, 2001·Journal of Clinical Microbiology·M DrancourtD Raoult
Feb 9, 2010·Bioscience, Biotechnology, and Biochemistry·Jin-Zhong XiaoKeiji Iwatsuki
Aug 22, 2015·Expert Review of Anti-infective Therapy·Roland NauHelmut Eiffert
Oct 1, 2013·Expert Review of Anti-infective Therapy·Roland NauHelmut Eiffert
Jan 31, 2003·Expert Opinion on Investigational Drugs·David M RothsteinBarry I Eisenstein
Apr 14, 2009·International Journal of Food Microbiology·Akira KushiroAriane van de Moer
Apr 2, 2005·Biotechnology and Bioengineering·Lars M BlankLars K Nielsen
Sep 19, 2018·Nature Microbiology·Nicole C HowardShabaana A Khader
May 23, 2000·Journal of Dairy Science·S A Ibrahim, D J O'Sullivan
Mar 15, 2006·The Journal of Antimicrobial Chemotherapy·Stephanie K Henderson-BeggLucinda M C Hall
Oct 3, 1999·Antimicrobial Agents and Chemotherapy·T Padayachee, K P Klugman
May 29, 1999·Antimicrobial Agents and Chemotherapy·M HeepN Lehn
Oct 3, 1998·Antimicrobial Agents and Chemotherapy·H Aubry-DamonP Courvalin