DOI: 10.1101/482810Nov 29, 2018Paper

Molecular fingerprints for a novel glucosamine kinase family in Actinobacteria

BioRxiv : the Preprint Server for Biology
José A MansoPedro JB Pereira

Abstract

Actinobacteria have long been the main source of antibiotics, secondary metabolites with tightly controlled biosynthesis by environmental and physiological factors. Phosphorylation of exogenous glucosamine has been suggested as a mechanism for incorporation of this extracellular material into secondary metabolite biosynthesis, but experimental evidence of specific glucosamine kinases in Actinobacteria is lacking. Here, we present the molecular fingerprints for the identification of a unique family of actinobacterial glucosamine kinases. Structural and biochemical studies on a distinctive kinase from the soil bacterium Streptacidiphilus jiangxiensis unveiled its preference for glucosamine and provided structural evidence of a phosphoryl transfer to this substrate. Conservation of glucosamine-contacting residues across a large number of uncharacterized actinobacterial proteins unveiled a specific glucosamine-binding sequence motif. This family of kinases and their genetic context may represent the missing link for the incorporation of environmental glucosamine into the antibiotic biosynthesis pathways in Actinobacteria and can be explored to enhance antibiotic production.

Related Concepts

Antibiotics
Base Sequence
Environment
Glucosamine
Phosphorylation
Phosphotransferases
Binding Protein
Extracellular
Structure
Metabolite

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