Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells

Cell Death Discovery
Kanchalit ThanomkittiVisith Thongboonkerd

Abstract

Previous expression study using quantitative proteomics has shown that immune-mediated pathway may not be the main mechanism inducing alopecia areata (AA). Nevertheless, functional impact of such expression data set remained unknown and unexplored. This study thus aimed to define potentially novel mechanisms of the AA pathogenesis by functional investigations of the differentially expressed proteins previously identified from lesional biopsies. From 122 altered proteins, protein-protein interactions network analysis revealed that downregulated heat shock protein 90 (HSP90) and lamin A/C served as the central nodes of protein-protein interactions involving in several crucial biological functions, including cytoskeleton organization, extracellular matrix organization, and tissue development. Interaction between HSP90 and lamin A/C in dermal papilla cells (DPCs) was confirmed by reciprocal immunoprecipitation and immunofluorescence co-staining. Small-interfering RNA (siRNA) targeting to HSP90 (siHSP90) and lamin A/C (siLamin A/C) effectively reduced levels of HSP90 and lamin A/C, respectively and vice versa, comparing to non-transfected and siControl-transfected cells, strengthening their interactive roles in DPCs. Functional inve...Continue Reading

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Citations

Dec 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Erol C BayraktarWalter W Chen
Aug 20, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keerakarn SomsuanVisith Thongboonkerd
Aug 7, 2019·Journal of Experimental & Clinical Cancer Research : CR·Ziqiang ZhangJianjun Du

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Methods Mentioned

BETA
biopsies
immunoprecipitation
flow cytometry
transfection
Protein Assay
scraping

Software Mentioned

BD
[UNK]
Tarosoft® Image
ImageMaster 2D Platinum
Tarosoft ® Image framework
Tarosoft® Image framework
Search Tool for the Retrieval of ( STRING )

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