Molecular genetic analysis of Dichelobacter nodosus proteases AprV2/B2, AprV5/B5 and BprV/B in clinical material from European sheep flocks

Veterinary Microbiology
Anna StäubleJoachim Frey

Abstract

Dichelobacter nodosus, the etiological agent of ovine footrot, exists both as virulent and as benign strains, which differ in virulence mainly due to subtle differences in the three subtilisin-like proteases AprV2, AprV5 and BprV found in virulent, and AprB2, AprB5 and BprB in benign strains of D. nodosus. Our objective was a molecular genetic epidemiological analysis of the genes of these proteases by direct sequence analysis from clinical material of sheep from herds with and without history of footrot from 4 different European countries. The data reveal the two proteases known as virulent AprV2 and benign AprB2 to correlate fully to the clinical status of the individuals or the footrot history of the herd. In samples taken from affected herds, the aprV2 gene was found as a single allele whereas in samples from unaffected herds several alleles with minor modifications of the aprB2 gene were detected. The different alleles of aprB2 were related to the herds. The aprV5 and aprB5 genes were found in the form of several alleles scattered without distinction between affected and non-affected herds. However, all different alleles of aprV5 and aprB5 encode the same amino acid sequences, indicating the existence of a single protease ...Continue Reading

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Citations

Mar 7, 2015·Veterinary Microbiology·Luci A WitcombElizabeth M H Wellington
Jun 19, 2016·The Veterinary Record·G MaboniS Tötemeyer
Nov 5, 2016·The Veterinary Journal·Synnøve Vatn
Dec 30, 2014·Journal of Clinical Microbiology·George FilioussisJoachim Frey
May 10, 2017·Frontiers in Veterinary Science·Edward M SmithLaura E Green
Sep 30, 2017·Frontiers in Cellular and Infection Microbiology·Grazieli MaboniSabine Tötemeyer
Nov 7, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Deborah GreberAdrian Steiner
Apr 7, 2021·Journal of Wildlife Diseases·Gaia Moore-JonesMarie-Pierre Ryser-Degiorgis
Apr 13, 2021·The Veterinary Journal·Patrik ZanolariPeter Kuhnert

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