Molecular genetic analysis of easily accessible breast tumour DNA, purified from tissue left over from hormone receptor measurement

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
L L HansenT A Kruse

Abstract

In order to establish a large panel of normal and tumour DNA from primary breast cancer patients, we looked for a source of easily accessible, good quality breast tumour DNA. Following routine hormone receptor analysis at the hospital the leftover pellets contained the nuclei from the tumour tissue. We collected 670 pellets over a period of 2 1/4 years and isolated a large amount of DNA (on average 400 microg per pellet). To control the quality of this tumour DNA, we analysed 41 pellets and matching normal DNA for loss of heterozygosity (LOH), with 11 microsatellite markers along chromosome 17. This chromosome is well described for breast cancer. LOH is a sensitive method, requiring good quality and pure tumour DNA. Contamination with normal DNA will blur the results. We found a high rate of LOH, ranging from 33 to 74%, which is in agreement with other reports, and therefore recommend this rich source of breast tumour DNA for molecular biological analysis.

References

Aug 11, 1990·Nucleic Acids Research·J L WeberP E May
Aug 11, 1990·Nucleic Acids Research·J L WeberD H Ledbetter
Sep 1, 1985·Somatic Cell and Molecular Genetics·S BenchimolJ Peacock
Feb 11, 1988·Nucleic Acids Research·S A MillerH F Polesky
Nov 1, 1980·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)

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Citations

Jul 16, 2010·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Lasse Sommer KristensenLise Lotte Hansen
Feb 7, 2008·BioTechniques·Lise Lotte HansenCarsten Wiuf
Jun 4, 2008·Genes, Chromosomes & Cancer·Emilia WiechecLise Lotte Hansen
Dec 8, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Emilia WiechecLise Lotte Hansen

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