Molecular genetic analysis of plastocyanin biosynthesis in Chlamydomonas reinhardtii.

The Journal of Biological Chemistry
H H LiSabeeha Merchant

Abstract

Five plastocyanin-deficient mutants were identified from a population of UV-mutagenized Chlamydomonas reinhardtii cells. Genetic complementation experiments indicated that four mutants represented alleles at the PCY1 locus (pcy1-2, pcy1-3, pcy1-4, and pcy1-5). Sequence analysis confirmed that two strains, pcy1-2 and pcy1-3, carry a frameshift (-1) and a nonsense mutation, respectively, while strains pcy1-4 and pcy1-5 synthesize an extended protein as a result of read-through mutations at the stop codon. The C-terminal extension does not affect synthesis or processing of the pre-proteins, but the polypeptides are rapidly degraded after the second (lumenal) processing event. The frameshift mutation in pcy1-2 results in loss of Pcy1 mRNA, as noted previously for strain ac208 (pcy1-1), but the abundance of Pcy1 mRNA in strain pcy1-3, which carries a nonsense mutation at codon 26, is unaffected relative to wild-type cells. The decreased abundance of frameshifted Pcy1 mRNA is attributed to increased degradation rather than decreased synthesis, since the mRNAs can be stabilized by treatment of cells with cycloheximide or anisomycin. The fifth strain has a wild-type plastocyanin-encoding gene, but the strain accumulates apoplastocyanin...Continue Reading

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Citations

Jun 5, 2003·The Plant Cell·Toshiharu ShikanaiMarinus Pilon
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Sabeeha Merchant, Beth Welty Dreyfuss
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Elizabeth H Harris
Feb 12, 2011·The Plant Journal : for Cell and Molecular Biology·Janette KropatDavin Malasarn
Jun 13, 2006·Biochimica Et Biophysica Acta·Sabeeha S MerchantAimee M Terauchi
Feb 12, 1998·The Journal of Biological Chemistry·K InoueO A Sodeinde
Nov 13, 2002·The Journal of Biological Chemistry·Beth Welty DreyfussSabeeha Merchant
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·R G DragerD B Stern
Oct 24, 1998·Biochimica Et Biophysica Acta·M HipplerJ D Rochaix

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